盐酸多巴胺(DH)和D-谷氨酸(D-Glu)是重要的兴奋性神经递质,与中枢神经系统疾病密切相关。因此,开发灵敏、简便的传感器来精确监测这些神经递质的变化是至关重要的。在这里,通过低成本且毫不费力的一锅溶剂热法合成了掺杂g的碳点(Gd-CD)。这些CD对DH和D-Glu表现出快速和可靠的荧光和比色响应信号。有趣的是,由于Gd-CD可以与DH的酚羟基配位,因此DH可以选择性地猝灭Gd-CD的荧光。此外,由于D-Glu与Gd-CD-DH系统竞争协调形成更稳定的复合物,因此有效抑制了淬灭过程。在荧光模式下,设计的荧光传感器对DH在1至10μM范围内具有良好的线性关系,检测限低至1.26nM,荧光可以通过D-Glu选择性地恢复。以比色的方式,通过UV-Vis吸收光谱可以在1-15μM和1-1.50mM的范围内检测到DH和D-Glu,分别。此外,所提出的方法不仅可以轻松监测水溶液中的DH和D-Glu,也可用于检测细胞中的DH和D-Glu。幸运的是,在全水介质中成功证明了荧光和比色双读出和逻辑运算。因此,制备的Gd-CD具有成为疾病诊断领域中DH和D-Glu传感的有前途的纳米传感器的潜力。
Dopamine hydrochloride (
DH) and D-Glutamic acid (D-Glu) are important excitatory neurotransmitters, which are closely relative to central nervous system diseases. Therefore, it is critical to develop the sensitive and facile sensor to precisely monitor the changes of these neurotransmitters. Herein, the gadolinium-doped carbon dots (Gd-CDs) were synthesized by a low-cost and effortless one-pot solvothermal method. These CDs exhibited rapid and reliable fluorescent and colorimetric response signals towards DH and D-Glu. Interestingly, the fluorescence of Gd-CDs could be selectively quenched by
DH owing to the fact that the Gd-CDs could coordinate with phenolic hydroxyl groups of
DH. Moreover, the quench process was effectively inhibited because the D-Glu competitively coordinated with Gd-CDs-DH system to form a more stable complex. In fluorescence mode, the designed fluorescence sensor possessed an excellent linear relationship for
DH in the range from 1 to 10 μM with a low detection limit of 1.26 nM, and the fluorescence could be selectively recovered by D-Glu. In colorimetric manner,
DH and D-Glu could be detected by UV-Vis absorption spectrum in the range of 1-15 μM and 1-1.50 mM, respectively. Moreover, the proposed method could not only easily monitor the DH and D-Glu in aqueous solutions as well as mouse serum and human urine samples, but also be employed for detecting DH and D-Glu in cells. Fortunately, the fluorescent and colorimetric dual readout AND logic operation was successfully demonstrated in all-aqueous media. Accordingly, the prepared Gd-CDs hold the potential to become a promising nano-sensor for DH and D-Glu sensing in disease diagnosis areas.