背景:肌动蛋白与晶状体混浊有关;然而,与白内障有关的特定肌动蛋白相关途径仍未阐明。在这项研究中,肌动蛋白相关的蛋白质组变化和信号通路参与白内障的发展进行了评估。
方法:收集11例糖尿病性白内障(DC)患者白内障手术期间前囊和超声乳化(phaco)盒内容物,年龄相关性白内障(ARC)12例,和7例玻璃体切除术后白内障(PVC)患者。无目标,通过具有数据无关采集(DIA)的液相色谱-质谱法对蛋白质进行全局鉴定和定量.
结果:在phaco盒样本中,ARC中表达明显低于DC和PVC的蛋白质参与各种途径,包括肌动蛋白结合,肌动蛋白细胞骨架重组,肌动蛋白丝封盖,皮质肌动蛋白细胞骨架组织,和小的GTP酶介导的信号转导途径。在前囊中,ARC中表达明显低于DC和PVC的蛋白质参与肌动蛋白结合和肌动蛋白细胞骨架重组途径。
结论:肌动蛋白细胞骨架和肌动蛋白结合蛋白参与晶状体纤维的伸长和分化。RhoGTPases有助于肌动蛋白细胞骨架重组,它们的失活与晶状体纤维异常迁移有关。这些发现将肌动蛋白结合与晶状体纤维完整性联系起来,晶状体混浊,和白内障。
BACKGROUND: Actin has been implicated in lens opacification; however, the specific actin-related pathways involved in cataracts remain unelucidated. In this study, actin-related proteome changes and signaling pathways involved in the development of cataracts were evaluated.
METHODS: The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from 11 patients with diabetic cataract (DC), 12 patients with age-related cataract (ARC), and seven patients with post-vitrectomy cataract (PVC). Untargeted, global identification and quantification of proteins was performed through liquid chromatography-mass spectrometry with the data-independent acquisition (DIA).
RESULTS: In phaco cassette samples, proteins with significantly lower expression in ARC than in DC and PVC were involved in various pathways, including actin binding, actin
cytoskeleton reorganization, actin filament capping, cortical actin
cytoskeleton organization, and small GTPase-mediated signal transduction pathways. In anterior capsules, proteins with significantly lower expression in ARC than in DC and PVC were involved in actin binding and actin
cytoskeleton reorganization pathways.
CONCLUSIONS: Actin
cytoskeleton and actin-binding proteins are involved in lens fiber elongation and differentiation. Rho GTPases contribute to actin cytoskeletal reorganization, and their inactivation is linked to abnormal lens fiber migration. These findings link actin binding to lens fiber integrity, lens opacification, and cataracts.