背景:雾化给药地塞米松对中度哮喘马细胞因子调节的影响尚未被研究。
目的:观察雾化吸入地塞米松治疗中度哮喘马后炎性细胞因子mRNA表达的变化。
方法:患有自然发生的中度哮喘的马(n=16)和健康对照马(n=4)。在研究期间,所有马匹都被保持在尘土飞扬的环境中。
方法:前瞻性,平行,随机化,控制,盲法临床试验。血液内源性皮质醇,气管粘液,和支气管肺泡灌洗(BAL)在治疗前和治疗后13天,无论是雾化给予地塞米松(15mg,每日一次)或0.9%生理盐水(3mL).通过随机化函数(MicrosoftExcel)随机分配治疗组。BAL液中靶mRNA的扩增(IL-1β,IL-4、IL-5、IL-6、IL-8、IL-10、IL-12、IL-17、IL-23、IFN-γ、Eotaxin-2和TNF-α)通过qPCR获得,使用相对表达软件工具分析BAL炎性细胞因子mRNA。
结果:用地塞米松雾化给药治疗的马IL-5的相对表达增加(1.70倍),IL-6(1.71倍),IL-17(3.25倍),IL-12(1.66倍),和TNF-α(1.94倍),与在第0天收集的样品相比,在第14天收集的样品中IL-23的相对表达降低(1.76倍;P=.04)(所有P<.05)。用盐水雾化给药处理的马在任何基因的相对表达上都没有显着差异(均P>0.05)。
结论:雾化吸入地塞米松与炎性细胞因子mRNA表达增加有关。与地塞米松或盐水治疗相关的炎性气道细胞学没有改善。
BACKGROUND: Nebulized administration of dexamethasone on cytokine regulation in horses with moderate asthma has not been investigated.
OBJECTIVE: To investigate the changes in expression of inflammatory cytokine mRNA after nebulized administration of dexamethasone treatment of horses with moderate asthma.
METHODS: Horses with naturally occurring moderate asthma (n = 16) and healthy control horses (n = 4). All horses were kept in a dusty environment during the study.
METHODS: Prospective, parallel, randomized, controlled, blinded clinical trial. Blood endogenous cortisol, tracheal mucus, and bronchoalveolar lavage (BAL) were sampled before and after 13 days treatment with either nebulized administration of dexamethasone (15 mg once daily) or 0.9% saline (3 mL). Treatment groups were randomly allocated via randomization function (Microsoft Excel). Amplification of target mRNA in BAL fluid (IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-17, IL-23, IFN-γ, Eotaxin-2, and TNF-α) was achieved by qPCR, and the relative expression software tool was used to analyze BAL inflammatory cytokine mRNA.
RESULTS: Horses treated with nebulized administration of dexamethasone had increased relative expression of IL-5 (1.70-fold), IL-6 (1.71-fold), IL-17 (3.25-fold), IL-12 (1.66-fold), and TNF-α (1.94-fold), and decreased relative expression of IL-23 (1.76-fold; P = .04) in samples collected on Day 14, in comparison to samples collected on Day 0 (all P < .05). Horses treated with nebulized administration of saline had no significant difference in the relative expression of any gene (all P > .05).
CONCLUSIONS: Nebulized administration of dexamethasone was associated with increased expression of inflammatory cytokine mRNA. There was no improvement in inflammatory airway cytology associated with either dexamethasone or saline treatment.