隔膜样本是从Gifu县的53只梅花鹿中收集的,日本;分离了220个肌囊,在湿坐骑中检查,并根据其囊肿壁突起进行分类。然后对肌囊进行分子检查,以将它们分配给不同的物种。最初通过针对五个物种的cox1的多重PCR测定鉴定了220个肌囊中的11个,而其余11个肌囊通过标准PCR和测序鉴定。来自选择的肌囊的DNA用于cox1(59个序列)和18SrDNA(23个序列)的PCR扩增和测序。220个肌囊包含七个主要的cox1序列类型或物种。类型4和7被分配给已知物种毛囊孢子虫和卵卵结囊孢子虫,而类型1,3和5被认为代表三个新物种,为其命名日本肉孢子虫,已经提出了松树孢子虫和gjerdei孢子虫。2型和6型与tarandi和taeniata类结节虫最相似,分别,但不能明确地分配给这些物种。属于日本血吸虫的结囊是宏观的,有相当厚的指状突起,而其他六个物种的大多数肌囊都是微观的。S.cf.tarandi和S.matsuoae呈纺锤形,并具有薄薄的手指状囊肿壁突起。S.pilosa和S.gjerdei的结囊有相似的毛发状突起,而S.cf.taeniata表面光滑。日本血吸虫的结囊,S.pilosa,S、参见。塔兰迪,S.Gjerdei,松树,S、参见。在50例(94.3%)中发现了taeniata和卵圆虫,29(54.7%),22(41.5%),10(18.9%),8(15.1%),检查的53只梅花鹿中有6只(11.3%)和1只(1.9%),分别。开发了一种针对cox1的改进的多重PCR检测方法,通过这七个结节虫。在本研究中发现可以确定。
Samples of diaphragm were collected from 53 sika deer from Gifu Prefecture, Japan; 220 sarcocysts were isolated, examined in wet mounts and classified according to their cyst wall protrusions. The sarcocysts were then examined molecularly in order to assign them to different species. All but 11 of the 220 sarcocysts were initially identified by means of a multiplex PCR assay targeting cox1 of five species, whereas the remaining 11 sarcocysts were identified by standard PCR and sequencing. DNA from selected sarcocysts was used for PCR amplification and sequencing of cox1 (59 sequences) and 18S rDNA (23 sequences). The 220 sarcocysts comprised seven major cox1 sequence types or species. Types 4 and 7 were assigned to the known species Sarcocystis pilosa and Sarcocystis ovalis, whereas types 1, 3 and 5 were considered to represent three new species, for which the names Sarcocystis japonica, Sarcocystis matsuoae and Sarcocystis gjerdei have been proposed. Types 2 and 6 were most similar to Sarcocystis tarandi and Sarcocystis taeniata, respectively, but could not be unequivocally assigned to these species. Sarcocysts belonging to S. japonica were macroscopic with fairly thick finger-like protrusions, whereas most sarcocysts of the six other species were microscopic. Sarcocysts of S. cf. tarandi and S. matsuoae were spindle-shaped and possessed thin finger-like cyst-wall protrusions. Sarcocysts of S. pilosa and S. gjerdei had similar hair-like protrusions, whereas those of S. cf. taeniata had a smooth surface. Sarcocysts of S. japonica, S. pilosa, S. cf. tarandi, S. gjerdei, S. matsuoae, S. cf. taeniata and S. ovalis were found in 50 (94.3%), 29 (54.7%), 22 (41.5%), 10 (18.9%), 8 (15.1%), 6 (11.3%) and 1 (1.9%) of the 53 sika deer examined, respectively. An improved multiplex PCR assay targeting cox1 was developed, through which the seven Sarcocystis spp. found in the present study could be identified.