■家禽的血液寄生虫感染,比如疟原虫,对家禽业造成严重威胁,因为它们有可能造成经济损失。迄今为止,在印度尼西亚,对感染家禽的不同疟原虫物种的形态学和分子检测研究不足。因此,本研究旨在分析疟原虫的形态和分子特征。以及日惹三个地区蛋鸡疟原虫感染的几个诱发因素,印度尼西亚。
■从位于日惹三个地区的13个农场中收集了一百零五个蛋鸡的血液样本(Sleman,班图尔,和KulonProgo)在2022年9月至11月之间。对血液样品进行显微镜和聚合酶链反应(PCR)分析。使用基本的局部比对搜索工具进行测序以鉴定细胞色素b的核苷酸结构。使用MEGA-X软件进行疟原虫的系统发育分析。
■显微镜检查显示血液寄生虫感染阳性17/105(16.19%)。滋养体,红细胞meronts,在血液样本中发现了疟原虫的微配子细胞。根据形态学检查,样本中发现的物种接近于近核疟原虫。聚合酶链反应检查显示21/60样品对疟原虫呈阳性(35%)。从测序样品中鉴定的疟原虫物种被证明是近核疟原虫。泰国近核假单胞菌与样本密切相关(99.64%-100%),遗传距离为0%-1%。此外,年龄,人口,和笼型与疟原虫感染无显著相关。
■基于显微镜和PCR检查,在日惹三个地区发现的疟原虫是近核疟原虫。日惹三个地区的样本之间的遗传距离与泰国和日本的近核假单胞菌密切相关(0%-1%)。疟原虫感染与年龄无关,笼型,或人口。
UNASSIGNED: Blood parasite infections in poultry, such as Plasmodium, are a serious threat to the poultry industry due to their potential to cause economic losses. To date, there has been inadequate research on the morphological and molecular detection of the different Plasmodium species that infect poultry in Indonesia. Therefore, this study aimed to analyze the morphological and molecular characteristics of Plasmodium spp. and the several predisposing factors for Plasmodium infection in layer chickens from three districts of Yogyakarta, Indonesia.
UNASSIGNED: One hundred and five blood samples from layer chickens were collected from 13 farms located in three districts of Yogyakarta (Sleman, Bantul, and Kulon Progo) between September and November 2022. Blood samples were subjected to microscopic and polymerase chain reaction (PCR) analyses. Sequencing was performed using basic local alignment search tools to identify the nucleotide structure of cytochrome b. Phylogenetic analysis of Plasmodium was performed using the MEGA-X software.
UNASSIGNED: Microscopic examination revealed that 17/105 positives (16.19%) were positive for blood parasite infection. Trophozoites, erythrocytic meronts, and microgametocytes of Plasmodium were found in blood samples. Based on the morphological examination, the species found in the samples was close to Plasmodium juxtanucleare. Polymerase chain reaction examination revealed that 21/60 samples were positive for Plasmodium (35%). The Plasmodium species identified from the sequenced samples were proven to be P. juxtanucleare. The P. juxtanucleare from Thailand was closely related to samples (99.64%-100%) with a genetic distance of 0%-1%. In addition, age, population, and cage type were not significantly associated with Plasmodium infection.
UNASSIGNED: Based on microscopic and PCR examinations, the Plasmodium species found in the three districts of Yogyakarta was P. juxtanucleare. The genetic distance between samples from the three districts of Yogyakarta was closely related (0%-1%) to P. juxtanucleare from Thailand and Japan. There was no correlation between Plasmodium infection and age, cage type, or population.