背景:Cymbopon是禾本科科的成员,已经对其植物化学物质和生物活性进行了探索。尽管Cymbogonspp的抗菌活性。提取物已被广泛研究,需要进行综合分析,以确定用于治疗抗微生物药物耐药性的有希望的化合物。因此,本研究调查了大红的抗氧化和抗菌性能。每个器官的乙醇提取物。
方法:乙醇提取物是从三种印度尼西亚商品的Cymbogonspp中获得的。,即Cymboponcitratus(L.)Rendle,Cymboponnardus(DC。)斯帕特。,和CymboponwinterianusJewitt。叶子,茎,和根提取物通过使用气相色谱-质谱(GC-MS)的代谢物谱分析进行评估。使用计算机模拟和体外分析来评估Cymbogonspp的抗氧化和抗菌性能。乙醇提取物。此外,使用细胞毒性测定法测量生物活性。使用1,1-二苯基-2-吡啶酰肼(DPPH)和2,2-叠氮基-双[3-乙基苯并噻唑啉-6-磺酸(ABTS)进行抗氧化剂测定,以使用溴化四唑(MTT)测定法确定对Huh7it-1细胞的毒性。最后,这些提取物的抗菌活性进行了评估,对白色念珠菌,枯草芽孢杆菌,金黄色葡萄球菌,和大肠杆菌使用良好的扩散测定。
结果:GC-MS分析显示53种代谢物。其中,2,5-双(1,1-二甲基乙基)-苯酚(27.87%),α-cadinol(26.76%),和1,2-二甲氧基-4-(1-丙烯基)-苯(20.56%)是主要化合物。C.winterianus和C.nardus叶对DPPH和ABTS表现出最高的抗氧化活性,分别。相反,MTT法显示低细胞毒性。C.nardus叶提取物对大肠杆菌和金黄色葡萄球菌表现出最高的抗菌活性,而冬虫夏草茎提取物对B.substilis显示出最高的活性。此外,计算途径分析预测抗菌活性机制与抗氧化活性有关。
结论:这些发现表明叶片具有很强的抗氧化活性,而叶和茎均表现出良好的抗菌活性。此外,所有的Cymboponspp。乙醇提取物显示低毒性。这些发现为评估Cymbogonspp的临床安全性的未来研究奠定了基础。作为新的候选药物。
BACKGROUND: Cymbopogon is a member of the family Poaceae and has been explored for its phytochemicals and bioactivities. Although the antimicrobial activities of
Cymbopogon spp. extracts have been extensively studied, comprehensive analyses are required to identify promising compounds for the treatment of antimicrobial resistance. Therefore, this study investigated the antioxidant and antimicrobial properties of Cymbopogon spp. ethanolic extracts in every single organ.
METHODS: Ethanolic extracts were obtained from three Indonesian commercial species of Cymbopogon spp., namely
Cymbopogon citratus (L.) Rendle,
Cymbopogon nardus (DC.) Spatf., and
Cymbopogon winterianus Jowitt. The leaf, stem, and root extracts were evaluated via metabolite profiling using gas chromatography-mass spectrometry (GC-MS). In silico and in vitro analyses were used to evaluate the antioxidant and antimicrobial properties of the Cymbopogon spp. ethanolic extracts. In addition, bioactivity was measured using cytotoxicity assays. Antioxidant assays were performed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid (ABTS) to determine toxicity to Huh7it-1 cells using a tetrazolium bromide (MTT) assay. Finally, the antimicrobial activity of these extracts was evaluated against Candida albicans, Bacillus subtilis, Staphylococcus aureus, and Escherichia coli using a well diffusion assay.
RESULTS: GC-MS analysis revealed 53 metabolites. Of these, 2,5-bis(1,1-dimethylethyl)- phenol (27.87%), alpha-cadinol (26.76%), and 1,2-dimethoxy-4-(1-propenyl)-benzene (20.56%) were the predominant compounds. C. winterianus and C. nardus leaves exhibited the highest antioxidant activity against DPPH and ABTS, respectively. Contrastingly, the MTT assay showed low cytotoxicity. C. nardus leaf extract exhibited the highest antimicrobial activity against E. coli and S. aureus, whereas C. winterianus stem extract showed the highest activity against B. substilis. Furthermore, computational pathway analysis predicted that antimicrobial activity mechanisms were related to antioxidant activity.
CONCLUSIONS: These findings demonstrate that the leaves had strong antioxidant activity, whereas both the leaves and stems showed great antimicrobial activity. Furthermore, all
Cymbopogon spp. ethanolic extracts showed low toxicity. These findings provide a foundation for future studies that assess the clinical safety of Cymbopogon spp. as novel drug candidates.