■先前的研究表明,肾阳虚证腹泻会导致小肠内容物和粘膜微生物群的紊乱。然而,TMA-裂解酶(CutC)活性和TMAO与肾阳虚证腹泻的关系仍未探讨。因此,本研究探讨了盲肠微生物群与胆碱TMA-裂解酶(CutC)活性之间的关系,以及三甲胺氧化物(TMAO)之间的相关性,炎症指数,和CutC活动。
■20只SPF级雄性KM小鼠随机分为正常组(CN)和腹泻模型组(CD)。通过腺嘌呤联合黄叶给药建立腹泻小鼠模型。CutC活动,TMAO,白细胞介素-6(IL-6),检测肿瘤坏死因子-α(TNF-α)水平,并对盲肠内容物微生物群进行测序。
■14天后,腹泻发生在CD组。与CN组相比,CD组小肠CutC活性无明显变化,而盲肠中CutC的活性显着增加,和TMAO的水平,IL-6和TNF-α显著升高。Chao1指数,观察到的物种指数,香农指数,辛普森指数均呈下降趋势。细菌属水平的主要变化是Alistipes,肠纹肌,Desulfovibrio,拟杆菌,Candidatus_圣公会,和[Ruminococus]_torques_group。LEfSe分析的结果,随机森林分析和ROC曲线分析显示,Blautia,Negativibacillus,Paraprevotella,Harryflintia,Candidatus_Soleaferrea,anaerotruncus,镰刀菌,Colidestribacter,[Ruminococus]_torques_group,和拟杆菌作为CD组的特征细菌。相关分析显示,盲肠CutC活性与利氏杆菌呈显著负相关,与阴型芽孢杆菌和Paludicola呈显著正相关。TMAO水平与CutC活性和IL-6呈显著正相关。
■腹泻肾阳虚证显著影响生理状态,消化酶活性,CutC活动,TMAO水平,和小鼠的炎症反应。此外,盲肠微生物群的组成和功能发生变化,提示肾阳虚型腹泻对宿主肠道菌群平衡有重要影响。肾阳虚证腹泻的发生可能与肠道菌群失调有关,CutC活性增加,升高的TMAO水平,炎症因子水平升高。
UNASSIGNED: Previous studies have indicated that diarrhea with kidney-yang deficiency syndrome leads to a disorder of small intestine contents and mucosal microbiota. However, the relationship of TMA-lyase (
CutC) activity and TMAO with diarrhea with kidney-yang deficiency syndrome remains unexplored. Therefore, this study explores the relationship between cecal microbiota and choline TMA-lyase (
CutC) activity, as well as the correlation between trimethylamine oxide (TMAO), inflammatory index, and
CutC activity.
UNASSIGNED: Twenty SPF-grade male KM mice were randomly divided into the normal group (CN) and the diarrhea model group (CD). Diarrhea mouse models were established by adenine combined with Folium sennae administration.
CutC activity, TMAO, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) levels were detected, and the cecal content microbiota was sequenced.
UNASSIGNED: After 14 days, diarrhea occurred in the CD group. Compared with the CN group, there was no significant change in the activity of CutC in the small intestine of the CD group, while the activity of CutC in the cecum was significantly increased, and the levels of TMAO, IL-6, and TNF-α showed a significant increase. The Chao1 index, Observed_species index, Shannon index, and Simpson index all exhibited a decreasing trend. The main changes at the bacterial genus level were Alistipes, Enterorhabdus, Desulfovibrio, Bacteroides, Candidatus_Saccharimonas, and [Ruminococcus]_torques_group. The results of LEfSe analysis, random forest analysis and ROC curve analysis revealed Paludicola, Blautia, Negativibacillus, Paraprevotella, Harryflintia, Candidatus_Soleaferrea, Anaerotruncus, Oscillibacter, Colidextribacter, [Ruminococcus]_torques_group, and Bacteroides as characteristic bacteria in the CD group. Correlation analysis showed a significant negative correlation between cecal CutC activity and Ligilactobacillus, and a significant positive correlation with Negativibacillus and Paludicola. The level of TMAO was significantly positively correlated with
CutC activity and IL-6.
UNASSIGNED: Diarrhea with kidney-yang deficiency syndrome significantly affects the physiological status, digestive enzyme activity, CutC activity, TMAO levels, and inflammatory response in mice. Additionally, there are changes in the composition and function of cecal microbiota, indicating an important impact of diarrhea with kidney-yang deficiency syndrome on the host intestinal microbiota balance. The occurrence of diarrhea with kidney-yang deficiency syndrome may be associated with dysbiosis of intestinal microbiota, increased CutC activity, elevated TMAO levels, and heightened inflammatory factor levels.