血小板是终末分化的无核细胞,但它们仍然具有细胞样功能,甚至可以产生后代血小板。然而,到目前为止,血小板发芽的机制尚未阐明。这里,我们显示,当富血小板血浆(PRP)在37°C培养时,血小板出现孢子现象。当给定特定剪切力时,血小板的数量增加。发现AMP相关的信号通路,例如PKA和AMPK在孢子状态的血小板中被激活。同时,基因的mRNA表达水平,例如CNN3,CAPZB,DBNL,与PLS1骨架蛋白相关的KRT19和ESPN也发生了变化。此外,当我们使用AMPK激活剂AICAR(AI)治疗洗涤的血小板时,培养的血小板仍然会出现孢子现象。我们进一步证明,用Forskolin处理的洗涤血小板,PKA的激活剂,培养后不仅血小板发芽,而且AMPK也被激活。一起来看,这些数据表明,AMPK在血小板出芽和增殖过程中起关键作用,提出了解决临床血小板短缺问题的新策略。
什么是新的?在这项研究中,我们发现,当富血小板血浆(PRP)在37℃培养时,血小板出现孢子现象并增加。发现AMP相关的信号通路,例如PKA和AMPK在孢子状态的血小板中被激活。此外,我们发现PKA是AMPK的上游激酶。在血小板发芽和增殖的过程中,骨架蛋白PLS1及其相关基因的mRNA表达水平,例如CNN3,CAPZB,DBNL,KRT19和ESPN也发生了变化。影响是什么?我们的研究提出了解决临床血小板短缺问题的新策略。
Platelets are terminally differentiated anucleated cells, but they still have cell-like functions and can even produce progeny platelets. However, the mechanism of platelet sprouting has not been elucidated so far. Here, we show that when platelet-rich plasma(PRP) was cultured at 37°C, platelets showed a spore phenomenon. The number of platelets increased when given a specific shear force. It is found that AMP-related signaling pathways, such as PKA and AMPK are activated in platelets in the spore state. Meanwhile, the mRNA expression levels of genes, such as CNN3, CAPZB, DBNL, KRT19, and ESPN related to PLS1 skeleton proteins also changed. Moreover, when we use the AMPK activator AICAR(AI) to treat washed platelets, cultured platelets can still appear spore phenomenon. We further demonstrate that washed platelets treated with Forskolin, an activator of PKA, not only platelet sprouting after culture but also the AMPK is activated. Taken together, these data demonstrate that AMPK plays a key role in the process of platelet budding and proliferation, suggesting a novel strategy to solve the problem of clinical platelet shortage.
What is new? In this study, we showed that when platelet-rich plasma(PRP) was cultured at 37°C, platelets showed spore phenomenon and increased.It was found that AMP-related signaling pathways, such as PKA and AMPK were activated in platelets in the spore state.In addition, we found that PKA acts as an upstream kinase of AMPK.In the process of platelet sprouting and proliferation, the mRNA expression levels of skeleton protein PLS1 and its related genes, such as CNN3, CAPZB, DBNL, KRT19, andESPN also changed.What is the impact? Our study proposes a new strategy to solve the problem of clinical platelet shortage.