背景:猪带虫囊虫病的抗原检测证实了中间宿主(猪或人)中的可行感染。参考B158/B60单克隆抗体(mAb)为基础的Ag酶联免疫吸附测定(ELISA)在患有多发性脑囊肿的人神经囊虫病中具有可接受的灵敏度和特异性,尽管在单发脑囊肿的病例中,其敏感性较低,而在猪囊虫病中,检测特异性受到其与猪带虫的频繁交叉反应的影响,在猪身上发现的另一种常见的食宿。我们小组生产了21种抗T。对整个囊肿的抗原有反应的单克隆抗体,囊泡液体,和分泌/排泄产物,将TsW8/TsW5鉴定为用于Ag-ELISA的最有希望的mAb对。
方法:我们报告了使用TsW8/TsW5Ag-ELISA测量从感染后第0天(基线)至第90天(PI)从实验感染了猪的猪(n=26)和猪(n=12)收集的两组血清中的囊尾蚴抗原水平[以光密度(OD)值表示]。在基线以及第28天和第90天,我们使用Bland-Altman(BA)分析和Lin的一致性相关系数(CCC)来确定TsW8/TsW5和B158/B60Ag-ELISA之间的一致性。
结果:TsW8/TsW5Ag-ELISA能够有效地测量感染猪的循环抗原水平。与B158/B60Ag-ELISA获得的相似。在基线和第28天和第90天PI(92.3%,100%,100%,分别),并且还发现测定之间的log-OD高度一致(Lin'sCCC:分别为0.69、0.92和0.96,所有P<0.001)。在感染了包虫的猪身上,在BA分析中,几乎所有配对的log-OD差异都在LoA范围内,而测定之间的log-OD一致性在基线时较低(Lin'sCCC:0.24),但在第28天和第90天PI时增加(Lins'CCC:0.88和0.98,P<0.001)。
结论:TsW8/TsW5Ag-ELISA可识别猪猪囊虫囊虫病中的抗原,与B158/B60Ag-ELISA高度一致。然而,它的诊断用途受到与包虫病的交叉反应的阻碍,与其他基于mAb的Ag-ELISA一样。
BACKGROUND: Antigen detection in Taenia solium cysticercosis confirms viable infection in the intermediate host (either pig or human). The reference B158/B60 monoclonal antibody (mAb)-based Ag-enzyme-linked immunosorbent assay (ELISA) has acceptable levels of sensitivity and specificity in human neurocysticercosis with multiple brain cysts, although its sensitivity is lower in cases with single brain cysts, whereas in porcine cysticercosis the assay specificity is affected by its frequent cross-reaction with Taenia hydatigena, another common cestode found in pigs. Our group has produced 21 anti-T. solium mAbs reacting against antigens of the whole cyst, vesicular fluid, and secretory/excretory products, identifying TsW8/TsW5 as the most promising pair of mAbs for an Ag-ELISA.
METHODS: We report the use of the TsW8/TsW5 Ag-ELISA to measure cysticercus antigen levels [expressed as optical density (OD) values] in two panels of sera collected from day 0 (baseline) to day 90 postinfection (PI) from pigs experimentally infected with T. solium (n = 26) and T. hydatigena (n = 12). At baseline and on days 28 and 90 PI, we used Bland-Altman (BA) analysis and Lin\'s concordance correlation coefficients (CCC) to determine the concordance between the TsW8/TsW5 and the B158/B60 Ag-ELISA.
RESULTS: The TsW8/TsW5 Ag-ELISA was able to efficiently measure circulating antigen levels in T. solium-infected pigs, similar to that obtained with the B158/B60 Ag-ELISA. Almost all paired log-OD differences between assays were within the limits of agreement (LoA) in the BA analysis at baseline and on days 28 and 90 PI (92.3%, 100%, and 100%, respectively), and a high concordance of log-ODs between assays was also found (Lin\'s CCC: 0.69, 0.92, and 0.96, respectively, all P < 0.001). In pigs infected with T. hydatigena, almost all paired log-OD differences were within the LoA in the BA analysis, whereas the concordance of log-ODs between assays was low at baseline (Lin\'s CCC: 0.24) but increased on days 28 and 90 PI (Lins\' CCC: 0.88 and 0.98, P < 0.001).
CONCLUSIONS: The TsW8/TsW5 Ag-ELISA recognizes antigens in pigs with T. solium cysticercosis and is highly concordant with the B158/B60 Ag-ELISA. However, its diagnostic use is hampered by cross-reactions with T. hydatigena, as in other mAb-based Ag-ELISAs.