Abstract：
To investigate the biological characteristics of monoclonal antibodies (mAbs) against avian influenza virus (AIV) and the possible mechanism of AIV-related kidney injury. BALB/c mice were immunized with inactivated H5N1 AIV to prepare monoclonal antibody H5-32, and its subtype, titer and cross-reactivity with other influenza viruses were identified. The reactivity of monoclonal antibody with normal human tissue was analyzed by immunohistochemistry. Immunofluorescence and confocal laser scanning technique were used to detect the binding sites between mAb and human renal cortical cells, and Western blotting was used to detect the size of binding fragments. Immunohistochemical analysis confirmed that monoclonal antibody H5-32 cross-reacted with normal human kidney tissue. In human kidney, mAb H5-32 was localized in the cytoplasm of human renal tubular epithelial cells, and its binding fragment size was about 43 kDa. H5N1 AIV appears to bind to human renal tubular epithelial cells, which may be one of the mechanisms of kidney injury caused by AIV infection.
摘要：
探讨抗禽流感病毒(AIV)单克隆抗体(mAb)的生物学特性及AIV相关肾损伤的可能机制。用灭活的H5N1AIV免疫BALB/c小鼠制备单克隆抗体H5-32,确定了滴度和与其他流感病毒的交叉反应性。通过免疫组织化学分析单克隆抗体与正常人组织的反应性。免疫荧光和共聚焦激光扫描技术用于检测mAb与人肾皮质细胞之间的结合位点。和Western印迹用于检测结合片段的大小。免疫组织化学分析证实单克隆抗体H5-32与正常人肾组织交叉反应。在人类肾脏中，mAbH5-32定位于人肾小管上皮细胞的细胞质中，其结合片段大小约为43kDa。H5N1AIV似乎与人肾小管上皮细胞结合，这可能是AIV感染引起肾损伤的机制之一。
