伤口愈合是一个复杂的过程,由多种细胞类型之间的相互作用协调,包括角质形成细胞,成纤维细胞,内皮细胞,炎症细胞,和生物活性因子如细胞外基质(ECM)成分,生长因子,和细胞因子。慢性伤口表现出延迟的增殖期开始,减少血管生成,ECM合成受损,和持续的炎症反应。慢性伤口是全球医疗系统面临的主要挑战之一,医疗服务费用高。因此,研究加速伤口愈合的新方法至关重要。植物药被认为是通过加速上皮形成改善伤口愈合的潜在药物。胶原蛋白合成,和血管生成。这些天然化合物具有各种优点,包括可用性,易于应用,伤口管理效率高。本研究旨在研究藏红花或番红花的生物学效应(C.sativus)花瓣提取物对细胞存活的影响,迁移,使用MTT和血管生成,划痕和体外试管形成测定。此外,评价人真皮成纤维细胞(HDF)和人脐静脉内皮细胞(HUVEC)中I型胶原α1(COL1A1)和血管内皮生长因子(VEGF)的表达,分别。还监测了C.sativus提取物对糖尿病小鼠皮肤的影响。结果表明,落叶提取物促进了HDFs和HUVECs的活力和迁移。此外,C.sativus花瓣提取物通过在Matrigel基底膜基质上培养的HUVEC增强了管状结构的形成,表明其刺激血管生成的潜力。基因表达研究表明,C.sativus提取物通过上调COL1A1和VEGF增加伤口愈合,这是胶原蛋白沉积的关键因素,上皮化,和血管生成。组织学分析显示,C.sativus花瓣提取物增强血管和增加成纤维细胞和胶原蛋白合成的数量,在糖尿病小鼠中,与用eucerin和商业软膏治疗的伤口相比,最终加速伤口闭合。因此,C.sativuspetal提取物具有作为草药治疗以改善糖尿病伤口的愈合的潜力。
Wound healing is a complex process orchestrated by interactions between a variety of cell types, including keratinocytes, fibroblasts, endothelial cells, inflammatory cells, and bioactive factors such as extracellular matrix (ECM) components, growth factors, and cytokines. Chronic wounds exhibit delayed proliferative phase initiation, reduced angiogenesis, impaired ECM synthesis, and persistent inflammatory response. Chronic wounds are one of the main challenges to the healthcare system worldwide, with a high cost for medical services. Hence, investigation of new approaches to accelerate wound healing is essential. Phytomedicines are considered as potential agents for improving the wound healing by accelerating epithelization, collagen synthesis, and angiogenesis. These natural compounds have various advantages including availability, ease of application, and high effectiveness in wound managment. This study aimed to investigate the biological effects of saffron or
Crocus sativus L. (C. sativus) petal extract on cell survival, migration, and angiogenesis using MTT, scratch and in vitro tube formation assays. Moreover, the expression of collagen type I alpha 1 (COL1A1) and vascular endothelial growth factor (VEGF) were evaluated in human dermal fibroblasts (HDF)s and human umbilical vein endothelial cells (HUVEC)s, respectively. The effect of the C. sativus extract on the skin of diabetic mice was also monitored. The results showed that C. sativus petal extract promoted the viability and migration of HDFs and HUVECs. Moreover, C. sativus petal extract enhanced the formation of tube-like structures by HUVECs cultured on the Matrigel basement membrane matrix, indicating its potential to stimulate angiogenesis. Gene expression studies have shown the the C. sativus extract increases wound healing by upregulation of COL1A1 and VEGF, which are crucial factors involved in collagen deposition, epithelialization, and angiogenesis. Histological analysis revealed that C. sativus petal extract enhanced vascularity and increased the number of fibroblasts and collagen synthesis, ultimately accelerating wound closure compared to wounds treated with eucerin and commercial ointment in diabetic mice. Therefore, C. sativus petal extract has potential as a herbal treatment to improve the healing of diabetic wounds.