■肠出血性大肠杆菌(O157:H7)是出血性腹泻或出血性结肠炎的主要原因。这项研究是为了确定亚的斯亚贝巴AkakiKaliti市乳制品供应链中O157:H7大肠杆菌的流行病学和抗菌素耐药性模式,埃塞俄比亚中部的Bishoftu和Sulululta镇。
■采用随机抽样方法的横断面研究设计。因此,总共450份原料牛奶(294份),从奶牛场收集牛奶手拭子(65)和水(91)样品,牛奶收集中心和自助餐厅,并根据标准进行处理,以分离和鉴定大肠杆菌O157:H7。样品最初富含缓冲蛋白胨水,然后铺在山梨醇MacConkey琼脂上。因此,使用乳胶凝集试验,对疑似非山梨糖醇发酵菌落进行生化和血清学试验,确认为大肠杆菌.
■在检查的450个样品中,6.0%被大肠杆菌O157:H7污染。因此,9.89%的水,9.23%的挤奶器手拭子和4.08%的原料奶样品被病原体污染。此外,大肠杆菌O157:H7的患病率为7.79%,Akakikaliti子城市的6.21%和3.97%,苏鲁塔和毕索夫图镇,分别。Fisher精确分析的结果表明,病原体的发生与样品来源之间存在显着差异(p<0.05),使用的水源,取样材料和容器类型。该研究还显示,大肠杆菌O157:H7分离株对所测试的9种抗菌圆盘的耐药性水平不同,并且100%(n=27)的分离株显示出多药耐药性,包括2至7种抗菌药物。
■总而言之,这项研究表明,在奶制品供应链的牛奶样品中,大肠杆菌O157:H7的发生及其多重耐药特征,以及对公众健康和食品安全的风险.因此,建议从奶牛场到叉子的适当卫生习惯和合理的药物使用。
UNASSIGNED: Enterohemorrhagic Escherichia coli (O157:H7) is the primary cause of bloody diarrhea or hemorrhagic colitis. The study was carried out with to determine the epidemiology and antimicrobial resistance pattern of E. coli O157:H7 along the dairy supply chains in Akaki Kaliti sub-city of Addis Ababa, Bishoftu and Sululta towns of central Ethiopia.
UNASSIGNED: A cross-sectional study design with random sampling methods was employed. Thus, a total of 450 raw cow milk (294), milker hand swab (65) and water (91) samples were collected from dairy farms, milk collection centers and Cafeterias and processed according to the standards to isolate and identify E. coli O157:H7. The samples were initially enriched in buffered peptone water, then plated onto Sorbitol MacConkey agar. Consequently, the suspected non-sorbitol fermenting colonies were confirmed as E. coli biochemically and serological test using latex agglutination tests.
UNASSIGNED: Out of the total 450 samples examined, 6.0% were found to be contaminated by E. coli O157:H7. Accordingly, 9.89% of water, 9.23% of milker hand swab and 4.08% of raw milk samples were contaminated by the pathogen. Furthermore, the prevalence of E. coli O157:H7 was 7.79%, 6.21% and 3.97% in Akaki kaliti sub-city, Sululta and Bishoftu towns, respectively. The result of Fisher exact analysis revealed a significant difference observed (p < 0.05) between the occurrence of the pathogen and the source of sample, sources of water used, sampled material and type of containers. The study also revealed that varying level of resistance of E. coli O157:H7 isolates against nine antimicrobial discs tested and 100% (n = 27) of the isolates showed multidrug-resistance comprising from two up to seven antimicrobial drugs.
UNASSIGNED: In conclusion, this study has indicated the occurrence of E. coli O157:H7 and its multiple drug-resistant profiles in milk samples along the dairy supply chains and its risk to public health and food safety. Therefore, proper hygienic practices from dairy farms to fork and rational drug usage are recommended.