杂酚油,煤蒸馏的副产品,主要由难以降解的芳香族化合物组成,如多环芳烃,酚类化合物,和N-,S-,和O-杂环化合物。尽管它具有毒性和致癌性,它仍然经常被用来浸渍木材,这对浸渍木质材料的植物的土壤条件具有特别的负面影响。因此,一个快速的,有效,必须开发消除这种土壤中杂酚的生态友好技术。研究的重点是获得固定在聚氨酯泡沫(PUF)中的BjerkanderaadustaDSM3375菌丝体的制备。它含有1.10±0.09g(DW)/g载体的霉菌细胞。获得的酶制剂用于被杂酚油(2%w/w)污染的土壤的生物修复。结果表明,在5、10和15周的生物修复中,应用PUF固定化B.adustaDSM3375菌丝体,分别,从土壤中去除19%,30%和35%的杂酚油。15周后,73、79和72%的荧蒽降解水平,芘,和芴,分别,已经发生了。固定化细胞具有大规模研究的潜力,因为它们可以降解土壤中的杂酚油。
Creosote oil, a byproduct of coal distillation, is primarily composed of aromatic compounds that are difficult to degrade, such as polycyclic aromatic hydrocarbons, phenolic compounds, and N-, S-, and O-heterocyclic compounds. Despite its toxicity and carcinogenicity, it is still often used to impregnate wood, which has a particularly negative impact on the condition of the soil in plants that impregnate wooden materials. Therefore, a rapid, effective, and eco-friendly technique for eliminating the
creosote in this soil must be developed. The research focused on obtaining a preparation of Bjerkandera adusta DSM 3375 mycelium immobilized in polyurethane foam (PUF). It contained mold cells in the amount of 1.10 ± 0.09 g (DW)/g of the carrier. The obtained enzyme preparation was used in the bioremediation of soil contaminated with
creosote (2% w/w). The results showed that applying the PUF-immobilized mycelium of B. adusta DSM 3375 over 5, 10, and 15 weeks of bioremediation, respectively, removed 19, 30, and 35% of
creosote from the soil. After 15 weeks, a 73, 79, and 72% level of degradation of fluoranthene, pyrene, and fluorene, respectively, had occurred. The immobilized cells have the potential for large-scale study, since they can degrade
creosote oil in soil.