背景:tramtrack(ttk)的转录产物是一种重要的转录因子,在发育调控中起着许多作用,生物体的分化和染色体重组。除了果蝇外,很少有关于ttk在其他昆虫中的特定功能的研究报道。我们的目的是揭示ttk对雄性水稻害虫褐飞虱(BPH)的发育和求爱的影响,Nilaparvatalugens.
结果:在这项研究中,我们首先检测了ttk在BPH中的时空表达,然后用dsttk治疗BPH的第四个若虫。我们发现大多数人在成年前死亡,成人羽化率仅为18.89%。在注射dsttk的个体中未发现求爱行为。进一步研究表明,注射dsttk的个体求偶振动信号(CVS)431.3Hz的主要频率明显高于注射dsGFP的个体223Hz,女性成年人对431.3HzCVS几乎没有反应。
结论:我们发现,接受dsttk治疗的BPH四龄若虫中约有81%在成年前死亡,成功出现的成年人发出431.3Hz的CVS,而女性成年人对此没有反应并失去了求爱能力。这是关于ttk在稻飞虱中的功能的首次发现,并说明了ttk作为RNAi防治稻飞虱的靶标的潜力。©2024化学工业学会。
BACKGROUND: The transcription product of tramtrack (ttk) is an important transcription factor which plays many roles in the regulation of the development, differentiation and chromosome recombination of organisms. Few studies have been reported on the specific functions of ttk in other insects except Drosophila melanogaster. Our aims are to reveal the ttk effects on development and
courtship of male rice pest brown planthopper (BPH), Nilaparvata lugens.
RESULTS: In this study, we first assayed spatiotemporal expression of ttk in BPH, then treated the fourth nymphs of BPH with dsttk. We found most individuals died before emerging to adults, the adult eclosion rate was only 18.89%. No
courtship behavior was found in individuals injected with dsttk. Further research showed that the main frequency of
courtship vibration signal (CVS) 431.3 Hz in the individuals injected with dsttk was significantly higher than 223 Hz in the individuals injected with dsGFP, and female adults nearly had no response to the 431.3 Hz CVS.
CONCLUSIONS: We found that about 81% of the 4-instar nymphs of BPH treated with dsttk died before they emerged as adults, the successfully emerged adults emitted the 431.3 Hz CVS to which female adults did not respond and lost the ability of
courtship. This was first finding about the functions of ttk in rice planthopper and illustrated the potential of ttk as target for RNAi to control rice planthopper. © 2024 Society of Chemical Industry.