我们的目的是确定半合成海藻酸钠水凝胶及其与富血小板血浆(PRP)的组合对组织病理学的影响,生物化学,临床,和眼前节光学相干断层扫描(AS-OCT)数据。引起角膜的碱性化学烧伤。将受伤的大鼠随机分为五个相等的组,并用磷酸盐缓冲盐水(sham)局部治疗,富血小板血浆(PRP),0.5%柠檬酸钠,半合成海藻酸钠水凝胶,或PRP和水凝胶的组合(组合组)每天三次。角膜混浊程度(CO),角膜上皮染色(CES),角膜上皮缺损百分比(CEDP),纤毛充血程度(CH),新生血管形成大小(NVS),评估新生血管形成(NVE)的程度。AS-OCT在第9天进行,然后处死老鼠.进行组织学检查和酶联免疫吸附试验以检测角膜中IL-1β和MMP-9的浓度。两组在CEDP方面没有显着差异,CO,CES,CH,NVS,角膜碱烧伤后的第一天或NVE(p>0.05)。在最后一次考试中,PRP组的CO显著低于假手术组(p=0.044),而CO浓度在NVS方面相似(p>0.05)。同样,在组织MMP-9水平方面,组间差异无统计学意义(p>0.05)。然而,两组之间的组织IL-1β水平存在显着差异(p<0,001)。在PRP和组合组中,IL-1β水平明显低于假手术组(p=0.043,p=0.036)。PRP之间的上皮坏死有显著差异,在联合组中最低(p=0.003)。联合组上皮厚度最高(p=0.002)。CEDP在最后一次访视时两组间有显著差异(p=0.042)。联合组的上皮闭合率最快(p=0.026)。根据AS-OCT测量,组织MMP-9水平与角膜硬度之间以及组织MMP-9水平与角膜面积之间存在显着负相关(分别为p=0.012和p=0.027)。单独使用时,局部应用水凝胶并没有显着增强角膜伤口的愈合。然而,当与PRP结合使用时,它导致上皮闭合和新生血管形成的速率增加。与单独使用PRP相比,这种组合不会加剧炎症或角膜混浊。PRP管中的抗凝血剂柠檬酸盐溶液证明无效。PRP和水凝胶的协同使用可以增加上皮厚度并减少上皮坏死。通过AS-OCT图像处理促进了角膜伤口愈合评估的新参数的使用。
Our objective was to determine the effect of a semi-synthetic sodium alginate hydrogel and its combination with platelet-rich plasma (PRP) on histopathological, biochemical, clinical, and anterior segment optical coherence tomography (AS-OCT) data. Alkali chemical burn of the cornea was induced. Injured rats were randomly divided into five equal groups and topically treated with phosphate-buffered saline (sham), platelet-rich plasma (PRP), 0.5% sodium citrate, a semi-synthetic sodium alginate hydrogel, or a combination of PRP and hydrogel (combined group) three times daily. The degree of corneal opacity (CO), corneal epithelial staining (CES), percentage of corneal epithelial defects (CEDP), degree of ciliary hyperemia (CH), neovascularization size (NVS), and extent of neovascularization (NVE) were evaluated. AS-OCT was performed at nine days, and then rats were sacrificed. Histological examination and enzyme-linked immunosorbent assays were performed to detect the concentrations of IL-1β and MMP-9 in the cornea. There were no significant differences between the groups regarding CEDP, CO, CES, CH, NVS, or NVE on the first day after corneal alkali burn injury (p > 0,05). At the last examination, CO was significantly lower in the PRP group than in the sham group (p = 0,044), while the CO concentrations were similar in terms of NVS (p > 0,05). Similarly, in terms of tissue MMP-9 levels, there were no significant differences between groups (p > 0,05). However, there was a significant difference in tissue IL-1β levels between the groups (p < 0,001). In the PRP and combined groups, the level of IL-1β was significantly lower than that in the sham group (p = 0,043 and p = 0,036, respectively). There was a significant difference in epithelial necrosis between the PRP, and it was the lowest in the combined group (p = 0,003). Epithelial thickness was highest in the combined group (p = 0,002). CEDP was significantly different at the last visit between the groups (p = 0.042). The fastest epithelial closing rate was observed for the combined group (p = 0,026). There was a significant negative correlation between tissue MMP-9 levels and corneal solidity and between tissue MMP-9 levels and the corneal area according to the AS-OCT measurements (p = 0,012 and p = 0,027, respectively). When used alone, topical hydrogel application did not significantly enhance the healing of corneal wounds. However, when combined with PRP, it leads to an increased rate of epithelial closure and neovascularization. This combination did not exacerbate inflammation or corneal opacity compared to PRP alone. The anticoagulant citrate solution in the PRP tube did not prove effective. The synergistic use of PRP and hydrogel could enhance epithelial thickness and reduce epithelial necrosis. The use of new parameters for corneal wound healing assessment was facilitated through AS-OCT image processing.