目的:观察水凝胶敷料对大鼠神经营养性角膜炎的治疗作用。
方法:雄性Wistar大鼠,年龄42-56d,被随机分为对照,实验性的,和治疗组,每个由五只老鼠组成。实验组和治疗组进行双眼神经营养性角膜炎建模。建模成功后,治疗组使用聚乙烯醇和聚乙烯吡咯烷酮形成的生物医学水凝胶7d。眼刺激反应和角膜炎指数评分,Schirmer\'stest,泪膜破裂时间(BUT),荧光素钠染色,采用苏木精和伊红(HE)染色评价治疗效果。
结果:成功建立了严重眼神经损伤大鼠神经营养性角膜炎模型,以角膜炎为特征,眼部刺激,通过降低的BUT和Schirmer测试值测量的泪液分泌减少,角膜上皮丢失,和基质层中无序的胶原纤维。用水凝胶敷料处理后,模型眼的角膜炎评分和眼部刺激症状显著改善.虽然泪液分泌的恢复,根据Schirmer的测试,没有显示出统计学差异,但显著延长。荧光素染色证实治疗后角膜上皮损失程度降低。HE染色显示上皮和基质层均在一定程度上恢复了结构紊乱。
结论:水凝胶敷料可减少眼表刺激,改善泪膜稳定性,并通过在大鼠模型眼表维持湿润清洁的环境促进受损上皮细胞的修复和恢复。
OBJECTIVE: To investigate the therapeutic effects of hydrogel dressings on neurotrophic keratitis in rats.
METHODS: Male Wistar rats, aged 42-56d, were randomly divided into control, experimental, and treatment groups, each consisting of five rats. The experimental and treatment groups underwent neurotrophic keratitis modeling in both eyes. After successful modeling, biomedical hydrogels formed with polyvinyl alcohol and polyvinyl pyrrolidone were used in treatment group for 7d. Ocular irritation response and keratitis index scores, Schirmer\'s test, tear film break-up time (BUT), sodium fluorescein staining, and hematoxylin and eosin (HE) staining were used to evaluate the effectiveness of the treatment.
RESULTS: The neurotrophic keratitis model was successfully established in rats with severe ophthalmic nerve injury, characterized by keratitis, ocular irritation, reduced tear secretion measured by decreased BUT and Schirmer test values, corneal epithelial loss, and disorganized collagen fibers in the stromal layer. Following treatment with hydrogel dressings, significant improvements were observed in keratitis scores and ocular irritation symptoms in model eyes. Although the recovery of tear secretion, as measured by the Schirmer\'s test, did not show statistical differences, BUT was significantly prolonged. Fluorescein staining confirmed a reduction in the extent of corneal epithelial loss after treatment. HE staining revealed the restoration of the structural disorder in both the epithelial and stromal layers to a certain extent.
CONCLUSIONS: Hydrogel dressing reduces ocular surface irritation, improves tear film stability, and promotes the repair and restoration of damaged epithelial cells by maintaining a moist and clean environment on the ocular surface in the rat model.