Abstract：
Given that the corneal epithelium is situated on the outermost part of the eye, its functions can be influenced by external temperatures and chemical substances. This study aimed to elucidate the expression profile of chemosensory receptors in corneal epithelial cells and analyze their role in eye function regulation. A comprehensive analysis of 425 chemosensory receptors in human corneal epithelial cells-transformed (HCE-T) revealed the functional expression of TRPV4. The activation of TRPV4 in HCE-T cells significantly increased the expression of membrane-associated mucins MUC1, MUC4, and MUC16, which are crucial for stabilizing tear films, with efficacy comparable to the active components of dry eye medications. The present study suggests that TRPV4, which is activated by body temperature, regulates mucin expression and proposes it as a novel target for dry eye treatment.
摘要：
鉴于角膜上皮位于眼睛的最外层，它的功能可能受到外部温度和化学物质的影响。本研究旨在阐明化学感觉受体在角膜上皮细胞中的表达谱，并分析其在眼功能调节中的作用。对人角膜上皮细胞转化（HCE-T）中425个化学感受器受体的综合分析揭示了TRPV4的功能表达。TRPV4在HCE-T细胞中的激活显著增加了膜相关粘蛋白MUC1、MUC4和MUC16的表达，这对于稳定泪膜至关重要。功效与干眼症药物的活性成分相当。本研究表明，由体温激活的TRPV4，调节粘蛋白表达，并提出将其作为干眼治疗的新靶标。
