细菌碳水化合物活性酶(CAZymes)的分析大大有助于理解珊瑚共生体对外部环境的反应。这项研究探讨了漂白对珊瑚Favitessp中细菌及其CAZyes的影响。通过宏基因组测序。值得注意的是,主坐标分析(PCoA)揭示了漂白和未漂白珊瑚之间细菌群落的实质性差异。变形杆菌,放线菌,酸杆菌,拟杆菌,和氯氟,在珊瑚漂白过程中表现出值得注意的变化。漂白珊瑚中的CAZymes谱揭示了糖基转移酶(GTs)丰度的显着增加,表明多糖的生物合成增强。相反,漂白珊瑚中的其他CAZymes丰度显着减少。变形杆菌,拟杆菌,绿虫,和Planctomycetota在漂白珊瑚中对CAZymes表现出更大的贡献,红霉素,细胞器,Burkholderiales,Caulobacterales,微生物是主要贡献者。虽然酸细菌,放线菌,和Chlooflexi在未漂白的珊瑚中对CAZymes的贡献更高。细菌及其CAZymes的变化反映了珊瑚全虫在面对环境胁迫时的生态适应性。漂白事件引起的CAZymes成分变化可能会对珊瑚养分吸收和生态系统稳定性产生深远的影响。因此,了解CAZymes的动态变化对于评估珊瑚生态系统的健康和恢复潜力至关重要。
Analysis of bacterial carbohydrate-active enzymes (CAZymes) contributes significantly to comprehending the response exhibited by coral symbionts to the external environment. This study explored the impact of bleaching on the bacteria and their CAZymes in coral Favites sp. through metagenomic sequencing. Notably, principal coordinates analysis (PCoA) unveiles substantial difference in bacterial communities between bleached and unbleached corals. Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidota, and Chloroflexi, exhibit noteworthy alterations during coral bleaching. CAZymes profiles in bleached coral disclosed a significant increase in Glycosyltransferases (GTs) abundance, suggesting an intensified biosynthesis of polysaccharides. Conversely, there is a marked reduction in other CAZymes abundance in bleached coral. Proteobacteria, Bacteroidota, Chlorobi, and Planctomycetota exhibit greater contributions to CAZymes in bleached corals, with Rhodobacterales, Cytophagales, Burkholderiales, Caulobacterales, and Hyphomicrobiales being the main contributors. While Acidobacteria, Actinobacteria, and Chloroflexi demonstrate higher contributions to CAZymes in unbleached corals. The changes in bacteria and their CAZymes reflect the ecological adaptability of coral holobionts when facing environmental stress. The alterations in CAZymes composition caused by bleaching events may have profound impacts on coral nutrient absorption and ecosystem stability. Therefore, understanding the dynamic changes in CAZymes is crucial for assessing the health and recovery potential of coral ecosystems.