背景:黄芩(黄芩,SR)和黄连(黄连,CR)是用于治疗高脂血症(HLP)的许多中药配方中的经典草药对。作为药物对的有效成分,黄连素和黄芩苷以成分配伍的形式治疗HLP的作用和机制尚不清楚。
目的:探讨SR和CR两种成分配伍治疗HLP的机制。
方法:采用高脂饮食建立HLP模型。检测血清生化指标。检测转录组学和代谢组学。采用RT-PCR和WesternBlot分析RA对HLP治疗过程中Cyp4a家族表达的影响。
结果:小檗碱-黄芩苷(RA)治疗HLP有较好的疗效。RA可以显著降低HLP的体重和肝脏重量,降低总胆固醇(TC)水平,甘油三酯(TG),低密度脂蛋白(LDL-C),并增加高密度脂蛋白(HDL-C)的水平。通过转录组学分析,RA显著逆转Cyp4a10、Cyp4a12b、细胞色素P450家族4亚家族a(Cyp4a)中的Cyp4a31和Cyp4a32与HLP小鼠肝脏中的脂肪酸降解有关。脂肪酸检测结果显示RA能显著调节庚酸,EPA,肾上腺酸,DH-γ-亚麻酸,和HLP小鼠盲肠中的DPA。RA调控的Cyp4a家族基因与RA调控的多种脂肪酸亲密相干。RT-PCR证实RA可以调节HLP小鼠Cyp4amRNA的表达。WB还显示RA可以调节Cyp4a的蛋白表达水平。
结论:SR和CR的成分配伍能有效改善HLP小鼠的血脂水平,其机制可能与调节Cyp4a基因表达和影响脂肪酸降解有关,调节体内脂肪酸代谢水平。
BACKGROUND: Scutellaria baicalensis Georgi (Scutellariae Radix, SR) and
Coptis chinensis Franch (Coptidis Rhizoma, CR) is a classic herbal pair used in many Traditional Chinese Medicine formulations in the treatment of hyperlipidemia (HLP). As effective ingredients of the drug pair, the effects and mechanisms of berberine and baicalin in the treatment of HLP in the form of components compatibility are still unclear.
OBJECTIVE: To explore the mechanism of the components compatibility of SR and CR in the treatment of HLP.
METHODS: The HLP model was established by a high-fat diet. Serum biochemical indexes were detected. Transcriptomics and metabolomics were detected. RT-PCR and Western Blot were used to analyze the effect of RA on the expression of the Cyp4a family during the treatment of HLP.
RESULTS: Berberine-baicalin (RA) has a good effect in the treatment of HLP. RA can significantly reduce the body weight and liver weight of HLP, reduce the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL-C), and increase the level of high-density lipoprotein (HDL-C). Through transcriptomic analysis, RA significantly reversed the gene expression of Cyp4a10, Cyp4a12 b, Cyp4a31, and Cyp4a32 in cytochrome P450 family 4 subfamily a (Cyp4a) which related to fatty acid degradation in the liver of HLP mice. The results of fatty acid detection showed that RA could significantly regulate heptanoic acid, EPA, adrenic acid, DH-γ-linolenic acid, and DPA in the cecum of HLP mice. The Cyp4a family genes regulated by RA are closely related to a variety of fatty acids regulated by RA. RT-PCR confirmed that RA could regulate Cyp4a mRNA expression in HLP mice. WB also showed that RA can regulate the protein expression level of Cyp4a.
CONCLUSIONS: The components compatibility of SR and CR can effectively improve the blood lipid level of HLP mice, its mechanism may be related to regulating Cyp4a gene expression and affecting fatty acid degradation, regulating the level of fatty acid metabolism in the body.