背景:瓢虫,七分球球菌,一个重要的捕食者,被广泛用于控制蚜虫,粉虱,螨虫,蓟马,和鳞翅目害虫。滞育控制技术是延长七叶菜货架期和商业化的关键。脂肪积累是繁殖滞育的主要特征,但AKH信号在生殖滞育过程中作为脂质动员调节剂的功能尚不清楚.本研究旨在鉴定和表征AKH和AKHR基因,并阐明它们在生殖滞育中的作用。
结果:头部和脂肪体中CsAKH和CsAKHR的相对表达水平最高,分别,在滞育条件下显着降低,在发育阶段和组织(头部,中肠,脂肪的身体,和卵巢)。此外,注射JH后CsAKH和CsAKHR表达显著增加,但CsMet沉默显著抑制CsAKH和CsAKHR表达,而CsMet敲除阻断了JH的诱导作用。CsAKH和CsAKHR敲除显著降低了含水量,增加脂质储存,促进与脂质合成相关的基因的表达,同时显著阻断卵巢发育,在繁殖条件下,在C.septempectuctata中诱导叉头盒O(FOXO)基因表达。相比之下,注射AKH肽显著抑制FOXO表达,减少脂质储存,在滞育条件下,七叶菜的含水量增加。
结论:这些结果表明,CsAKH和CsAKHR参与了C.septempuctata滞育期间脂质积累和卵巢发育的调节,并为操纵C.septempunctata滞育提供了一个有希望的目标。本文受版权保护。保留所有权利。
BACKGROUND: The ladybeetle, Coccinella septempunctata, an important predator, is widely used to control aphids, whiteflies, mites, thrips, and lepidopteran pests. Diapause control technology is key to extending C. septempunctata shelf-life and commercialization. Lipid accumulation is a major feature of reproductive diapause, but the function of AKH signaling as a regulator of lipid mobilization in reproductive diapause remains unclear. This study aimed to identify and characterize AKH and AKHR genes, and clarify their functions in reproductive diapause.
RESULTS: The relative expression levels of CsAKH and CsAKHR were the highest in the head and fat body, respectively, and were significantly decreased under diapause conditions, both in developmental stages and tissues (head, midgut, fat body, and ovary). Furthermore, CsAKH and CsAKHR expression was increased significantly after juvenile hormone (JH) injection, but CsMet silencing significantly inhibited CsAKH and CsAKHR expression, whereas CsMet knockdown blocked the induction effect of JH. CsAKH and CsAKHR knockdown significantly reduced water content, increased lipid storage, and promoted the expression of genes related to lipid synthesis, but significantly blocked ovarian development, and induced forkhead box O (FOXO) gene expression in C. septempunctata under reproduction conditions. By contrast, injection of AKH peptide significantly inhibited FOXO expression, reduced lipid storage, and increased water content in C. septempunctata under diapause conditions.
CONCLUSIONS: These results indicate that CsAKH and CsAKHR are involved in the regulation of lipid accumulation and ovarian development during diapause in C. septempunctata, and provide a promising target for manipulating C. septempunctata diapause. © 2024 Society of Chemical Industry.