■不孕症和多囊卵巢综合征(PCOS)的发病机制均受胰岛素抵抗和血脂异常的影响。大概,在这些患者的饮食中添加辅酶Q10(CoQ10)将是有益的。因此,本研究旨在研究补充辅酶Q10对体外受精(IVF)候选女性代谢谱的影响.
■对于此随机化,双盲,平行,安慰剂对照临床实验,包括40名IVF候选人的PCOS阳性不育妇女。他们的年龄从18岁到40岁不等。两个干预组中的20名参与者接受了CoQ10或安慰剂8周。葡萄糖转运蛋白1(GLUT-1)的表达,过氧化物酶体增殖物激活受体γ(PPAR-γ),低密度脂蛋白受体(LDLR),以及代谢谱,如胰岛素代谢和血脂谱进行了评估。定量RT-PCR检测GLUT-1、PPAR-γ、和外周血单核细胞上的LDLR。使用酶试剂盒评估血脂和空腹血糖,使用ELISA试剂盒测定胰岛素。
■与安慰剂相比,补充辅酶Q10可显着降低血液胰岛素水平(-0.3±1.0vs.0.5±0.7,P=0.01)和胰岛素抵抗(-0.1±0.2vs.0.1±0.2,P=0.01),PPAR-γ表达增加(P=0.01)。在不孕PCOS患者中,IVF的候选人,补充辅酶Q10对其他代谢谱没有明显影响。此外,补充辅酶Q10对GLUT-1无显著影响(P=0.30),或LDLR(P=0.27)表达。安慰剂组胰岛素水平(P=0.01)和胰岛素抵抗(P=0.01)的组内变化显着升高。当我们调整基线BMI的分析时,变量的基线值,和年龄,我们的发现没有受到影响.
■补充CoQ10八周对PPAR-γ表达显示出积极的益处,胰岛素抵抗,和血清胰岛素在不孕PCOS妇女候选人IVF。
UNASSIGNED: Infertility and the pathogenesis of polycystic ovarian syndrome (PCOS) are both influenced by insulin resistance and dyslipidemia. Presumably, adding coenzyme Q10 (CoQ10) to these patients\' diets will be beneficial. Therefore, this study aimed to examine the effects of CoQ10 supplementation on metabolic profiles in women candidates for in-vitro fertilization (IVF).
UNASSIGNED: For this randomized, double-blinded, parallel, placebo-controlled clinical experiment, 40 PCOS-positive infertile women who were IVF candidates were included. They ranged in age from 18 to 40. The 20 participants in the two intervention groups received either CoQ10 or a placebo for 8 weeks. The expression of glucose transporter 1 (GLUT-1), peroxisome proliferator-activated receptor gamma (PPAR-γ), low-density lipoprotein receptor (LDLR), as well as metabolic profiles such as insulin metabolism and lipid profiles were evaluated. Quantitative RT-PCR determined the expression of GLUT-1, PPAR-γ, and LDLR on peripheral blood mononuclear cells. Lipid profiles and fasting glucose were assessed using enzymatic kits, and insulin was determined using Elisa kit.
UNASSIGNED: In comparison to the placebo, CoQ10 supplementation significantly reduced blood insulin levels (-0.3±1.0 vs. 0.5±0.7, P=0.01) and insulin resistance (-0.1±0.2 vs. 0.1±0.2, P=0.01), and increased PPAR-γ expression (P=0.01). In infertile PCOS patients\' candidates for IVF, CoQ10 supplementation showed no appreciable impact on other metabolic profiles. Also, CoQ10 supplementation revealed no significant impact on GLUT-1 (P=0.30), or LDLR (P=0.27) expression. Within-group changes in insulin levels (P=0.01) and insulin resistance (P=0.01) showed a significant elevation in the placebo group. When we adjusted the analysis for baseline BMI, baseline values of variables, and age, our findings were not affected.
UNASSIGNED: Eight weeks of CoQ10 supplementation demonstrated positive benefits on PPAR-γ expression, insulin resistance, and serum insulin in infertile PCOS women candidates for IVF.