肥胖及其并发症对全球人类健康构成主要威胁。本研究的目的是探讨酪酸梭菌(Ct)对脂质代谢的影响,肠屏障功能,高脂饮食(HFD)诱导的肥胖小鼠的肠道微生物组。建立肥胖模型后,107CFU/mL和108CFU/mL的酪丁酸梭菌通过管饲法干预喂养的HFD小鼠6周,并测量与肥胖相关的指标。在HFD喂养小鼠的肝脏中,结果显示,酪丁酸梭菌可降低肝脏重量和甘油三酯(TG)水平,总胆固醇(TC),和非酯化脂肪酸(NEFA),随着红色脂滴和脂肪空泡的减少。酪氨酸丁酸梭菌干预后,过氧化物酶体增殖物激活受体γ(PPARγ)mRNA表达下调,和AMP激活的蛋白激酶(AMPK),过氧化物酶体增殖物激活受体-α(PPARα),脂肪甘油三酯脂肪酶(ATGL),和激素敏感脂肪酶(HSL)在肝脏中上调。此外,酪丁酸梭菌减轻HFD引起的肠道形态损伤,降低肿瘤坏死因子-α(TNF-α)的表达,白细胞介素6(IL-6),和结肠中的IL-1β,并上调紧密连接蛋白的表达。此外,16SrRNA测序显示,酪丁酸梭菌增加了肠道微生物群的多样性。总的来说,酪丁酸梭菌改善HFD诱导的脂质代谢紊乱,保留了肠道屏障的完整性,并调节肠道微生物组的结构。这些发现提供了一种新的了解作为益生菌在调节脂质代谢中的作用。
Obesity and its complications constitute a main threat to global human health. The purpose of this investigation was to explore the influences of Clostridium tyrobutyricum (Ct) on lipid metabolism, intestinal barrier function, and intestinal microbiome in obese mice induced by a high-fat diet (HFD). After establishing the obesity model, 107 CFU/mL and 108 CFU/mL C. tyrobutyricum were used to intervene in HFD-fed mice by gavage for six weeks, and indexes related to obesity were measured. In the liver of HFD-fed mice, the results revealed that C. tyrobutyricum reduced liver weight and the levels of triglyceride (TG), total cholesterol (TC), and nonesterified fatty acid (NEFA), along with decreasing red lipid droplets and fat vacuoles. After C. tyrobutyricum intervention, the mRNA expression of peroxisome proliferator-activated receptor-γ (PPARγ) was downregulated, and AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor-α (PPARα), adipose triglyceride lipase (ATGL), and hormone-sensitive lipase (HSL) were upregulated in the liver. Additionally, C. tyrobutyricum alleviated intestinal morphology injury caused by HFD, decreased the expression of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and IL-1β in the colon, and upregulated tight junction protein expression. In addition, 16S rRNA sequencing revealed that C. tyrobutyricum increases the diversity of intestinal microbiota. Overall, C. tyrobutyricum improved HFD-induced lipid metabolism disorders, preserved the intestinal barrier\'s integrity, and modulated the structure of the intestinal microbiome. These findings provide a novel insight into the role of C. tyrobutyricum as a probiotic in regulating lipid metabolism.