背景:宫颈癌是女性普遍存在的致命恶性肿瘤,由于明显的副作用和化疗耐药性的发展,化疗往往被证明是无效的。这项研究调查了Clerodendruminfortinatumlinn的药用潜力。,在唇形科中大约有500种的属。对石竹种类及其各种溶剂提取物的研究有限。
目的:该研究旨在评估该植物的不同溶剂提取物对人宫颈癌细胞的抗癌特性。
方法:该研究检查了植物的植物化学成分及其抑制癌症生长的潜力。使用索氏方法提取植物的地上部分,还有芦丁的存在,槲皮素,通过高效薄层色谱法(HPTLC)验证特定溶剂提取物中的没食子酸和没食子酸。体外试验,包括MTT,细胞凋亡,细胞周期分析,细胞内活性氧物种评估,和基因表达PCR,进行了进一步研究植物的抗癌特性。
结果:植物化学评估的结果表明芦丁主要存在于水提取物中,槲皮素在汤剂中更集中,和水醇提取物显示出升高的没食子酸水平。值得注意的是,汤提取物表现出最高的细胞毒活性,主要通过S期和G2M期的早期凋亡和停滞。厄运克罗根鼓表现出细胞内活性氧的减少。基因表达分析揭示了对BCL-2基因的影响。
结论:值得注意的是,Clerodendruminflohatum表现出启动早期凋亡的能力,在S期和G2M期停止细胞周期,并显著降低活性氧的水平。基因表达分析揭示了对BCL-2基因的影响。总而言之,这项研究强调了Clerodendruminfortunatum的令人鼓舞的细胞毒性和抗氧化特性,暗示其治疗宫颈癌的潜力。
BACKGROUND: Cervical cancer is a prevalent and deadly malignancy in females, with chemotherapy often proving ineffective due to significant side effects and the development of chemo-resistance. This study investigates the medicinal potential of
Clerodendrum infortunatum linn. , a genus with approximately 500 species in the Lamiaceae family. Limited research exists on the species of
Clerodendrum infortunatum and its various solvent extracts.
OBJECTIVE: The study aims to assess the anti-cancer properties of different solvent extracts from this plant on human cervical cancer cells.
METHODS: The study examines the plant\'s phytochemical components and their potential to inhibit cancer growth. Aerial parts of the plant were extracted using the Soxhlet method, and the presence of Rutin, Quercetin, and Gallic Acid in specific solvent extracts was validated through High-Performance Thin Layer Chromatography (HPTLC). In vitro assays, including MTT, Apoptosis, Cell Cycle analysis, Intracellular Reactive Oxygen Species assessment, and Gene expression PCR, were conducted to investigate the plant\'s anti-cancer properties further.
RESULTS: The outcomes of the phytochemical assessment indicated that Rutin was predominantly present in the water extract, with quercetin being more concentrated in the decoction, and the hydro-alcoholic extract showing elevated levels of gallic acid. Notably, the decoction extract demonstrated the highest cytotoxic activity, primarily through early apoptosis and arrests in the S-phase and G2M phases.
Clerodendrum infortunatum exhibited a reduction in Intracellular Reactive Oxygen Species. The gene expression analysis disclosed an impact on the BCL-2 gene.
CONCLUSIONS: Notably,
Clerodendrum infortunatum exhibited the ability to initiate early apoptosis, halt the cell cycle at the S and G2M phases, and diminish levels of reactive oxygen species significantly. The gene expression analysis revealed an influence on the BCL-2 gene. To sum up, this research underscores the encouraging cytotoxic and antioxidant attributes of
Clerodendrum infortunatum, implying its potential for cervical cancer treatment.