METHODS: Leaves of C. myricoides were extracted using the maceration technique. The extract was formulated as 5% and 10% w/w ointments. The wound healing activity of the extract was evaluated using excision, incision, and burn wound models whereas the healing activities of solvent fractions were evaluated using the excision wound model. A carrageenan-induced paw edema model was used for the anti-inflammatory test.
RESULTS: In the dermal toxicity test, 2000 mg/kg of 10% extract was found to be safe. In excision and burn wound models, treatment with 10% and 5% extract showed a significant (p<0.001) wound contraction. Solvent fractions of the extract significantly reduced wound contraction. A significant reduction in periods of epithelialization and favorable histopathology changes were shown by extract ointments. In incision wounds, 10% (p<0.001) and 5% (p<0.01) extracts significantly increase skin-breaking strength. After one hour of treatment, 400 mg/kg (p<0.001) and 200 mg/kg (p<0.05) showed significant reduction in paw edema.
CONCLUSIONS: Results of this study indicate that 80% methanol extract and the solvent fraction of the leaves of C. myricoides possess wound-healing and anti-inflammatory activity and support traditional claims.
方法:使用浸渍技术提取木耳梭菌的叶。将提取物配制为5%和10%w/w软膏。使用切除评估提取物的伤口愈合活性,切口,和烧伤伤口模型,而溶剂部分的愈合活性使用切除伤口模型进行评估。角叉菜胶诱导的爪水肿模型用于抗炎试验。
结果:在皮肤毒性试验中,发现2000mg/kg的10%提取物是安全的。在切除和烧伤伤口模型中,用10%和5%提取物处理显示显著(p<0.001)的伤口收缩。提取物的溶剂部分显著降低了伤口收缩。提取物软膏显示上皮形成时间的显着减少和有利的组织病理学变化。在切口伤口中,10%(p<0.001)和5%(p<0.01)提取物显着增加皮肤断裂强度。治疗一小时后,400mg/kg(p<0.001)和200mg/kg(p<0.05)显示爪水肿显著减少。
结论:这项研究的结果表明,80%的甲醇提取物和Myricoides叶的溶剂部分具有伤口愈合和抗炎活性,并支持传统的主张。