PDF(Pubmed)
Abstract：
BACKGROUND: Wounds significantly affect people\'s quality of life and the clinical and financial burden of healthcare systems around the world. Many of the current drugs used to treat wounds have problems such as; allergies and drug resistance. Hence, the exploration of new therapeutic agents from natural origin may avert this problem. Clerodendrum myricoides have long been used to treat wounds in Ethiopia. Despite this, nothing has so far been reported about the wound healing and anti-inflammatory activity of C. myricoides. This study aimed to evaluate the wound healing and anti-inflammatory activity of 80% methanol extract and solvent fractions of C. myricoides leaves in mice.
METHODS: Leaves of C. myricoides were extracted using the maceration technique. The extract was formulated as 5% and 10% w/w ointments. The wound healing activity of the extract was evaluated using excision, incision, and burn wound models whereas the healing activities of solvent fractions were evaluated using the excision wound model. A carrageenan-induced paw edema model was used for the anti-inflammatory test.
RESULTS: In the dermal toxicity test, 2000 mg/kg of 10% extract was found to be safe. In excision and burn wound models, treatment with 10% and 5% extract showed a significant (p<0.001) wound contraction. Solvent fractions of the extract significantly reduced wound contraction. A significant reduction in periods of epithelialization and favorable histopathology changes were shown by extract ointments. In incision wounds, 10% (p<0.001) and 5% (p<0.01) extracts significantly increase skin-breaking strength. After one hour of treatment, 400 mg/kg (p<0.001) and 200 mg/kg (p<0.05) showed significant reduction in paw edema.
CONCLUSIONS: Results of this study indicate that 80% methanol extract and the solvent fraction of the leaves of C. myricoides possess wound-healing and anti-inflammatory activity and support traditional claims.
METHODS: Leaves of C. myricoides were extracted using the maceration technique. The extract was formulated as 5% and 10% w/w ointments. The wound healing activity of the extract was evaluated using excision, incision, and burn wound models whereas the healing activities of solvent fractions were evaluated using the excision wound model. A carrageenan-induced paw edema model was used for the anti-inflammatory test.
RESULTS: In the dermal toxicity test, 2000 mg/kg of 10% extract was found to be safe. In excision and burn wound models, treatment with 10% and 5% extract showed a significant (p<0.001) wound contraction. Solvent fractions of the extract significantly reduced wound contraction. A significant reduction in periods of epithelialization and favorable histopathology changes were shown by extract ointments. In incision wounds, 10% (p<0.001) and 5% (p<0.01) extracts significantly increase skin-breaking strength. After one hour of treatment, 400 mg/kg (p<0.001) and 200 mg/kg (p<0.05) showed significant reduction in paw edema.
CONCLUSIONS: Results of this study indicate that 80% methanol extract and the solvent fraction of the leaves of C. myricoides possess wound-healing and anti-inflammatory activity and support traditional claims.
摘要：
背景：创伤显著影响人们的生活质量以及世界各地医疗保健系统的临床和经济负担。许多目前用于治疗伤口的药物具有诸如过敏和耐药性的问题。因此，从天然来源探索新的治疗药物可以避免这个问题。Clerodendrummyricoides长期以来一直用于治疗埃塞俄比亚的伤口。尽管如此,到目前为止，还没有关于Myricoides的伤口愈合和抗炎活性的报道。本研究旨在评估80％甲醇提取物和木耳梭菌叶的溶剂部分在小鼠中的伤口愈合和抗炎活性。
方法:使用浸渍技术提取木耳梭菌的叶。将提取物配制为5%和10%w/w软膏。使用切除评估提取物的伤口愈合活性，切口,和烧伤伤口模型，而溶剂部分的愈合活性使用切除伤口模型进行评估。角叉菜胶诱导的爪水肿模型用于抗炎试验。
结果：在皮肤毒性试验中，发现2000mg/kg的10%提取物是安全的。在切除和烧伤伤口模型中，用10%和5%提取物处理显示显著(p<0.001)的伤口收缩。提取物的溶剂部分显著降低了伤口收缩。提取物软膏显示上皮形成时间的显着减少和有利的组织病理学变化。在切口伤口中，10％（p<0.001）和5％（p<0.01）提取物显着增加皮肤断裂强度。治疗一小时后，400mg/kg(p<0.001)和200mg/kg(p<0.05)显示爪水肿显著减少。
结论：这项研究的结果表明，80％的甲醇提取物和Myricoides叶的溶剂部分具有伤口愈合和抗炎活性，并支持传统的主张。
方法:使用浸渍技术提取木耳梭菌的叶。将提取物配制为5%和10%w/w软膏。使用切除评估提取物的伤口愈合活性，切口,和烧伤伤口模型，而溶剂部分的愈合活性使用切除伤口模型进行评估。角叉菜胶诱导的爪水肿模型用于抗炎试验。
结果：在皮肤毒性试验中，发现2000mg/kg的10%提取物是安全的。在切除和烧伤伤口模型中，用10%和5%提取物处理显示显著(p<0.001)的伤口收缩。提取物的溶剂部分显著降低了伤口收缩。提取物软膏显示上皮形成时间的显着减少和有利的组织病理学变化。在切口伤口中，10％（p<0.001）和5％（p<0.01）提取物显着增加皮肤断裂强度。治疗一小时后，400mg/kg(p<0.001)和200mg/kg(p<0.05)显示爪水肿显著减少。
结论：这项研究的结果表明，80％的甲醇提取物和Myricoides叶的溶剂部分具有伤口愈合和抗炎活性，并支持传统的主张。
