背景:同源异型盒基因在牙齿形态发生和发育中起关键作用,因此同源异型盒基因的突变会导致发育障碍,例如牙源性病变。这项范围审查的目的是从有关牙源性病变中同源盒基因表达的文献中鉴定和汇编可用数据。
方法:在四个数据库(PubMed,EBSCO主机,WebofScienceandCochraneLibrary)withselected关键字.考虑了所有报道同源盒基因在牙源性病变中表达的论文。
结果:共鉴定了11篇描述同源异型盒基因在牙源性病变中表达的论文。研究方法包括下一代测序,微阵列分析,RT-PCR,西方印迹,原位杂交,和免疫组织化学。牙源性病变中报告的同源盒包括牙瘤中的LHX8和DLX3;PITX2,MSX1,MSX2,DLX,成釉细胞瘤中的DLX2,DLX3,DLX4,DLX5,DLX6,ISL1,OCT4和HOXC;腺瘤样牙源性肿瘤中的OCT4;原始牙源性肿瘤中的PITX2和MSX2;牙源性角化囊肿中的PAX9和BARX1;DLamilaboric癌中的PITX2,ZEIS2和MEIS2,而无牙源性细胞
结论:本文对同源盒基因表达在牙源性病变中的可能联系进行了总结和综述。根据当前可用的数据,没有足够的证据支持同源异型盒基因在牙源性病变中的明确作用。
BACKGROUND: Homeobox genes play crucial roles in tooth morphogenesis and development and thus mutations in homeobox genes cause developmental disorders such as odontogenic lesions. The aim of this scoping review is to identify and compile available data from the literatures on the topic of homeobox gene expression in odontogenic lesions.
METHODS: An electronic search to collate all the information on studies on homeobox gene expression in odontogenic lesions was carried out in four databases (PubMed, EBSCO host, Web of Science and Cochrane Library) with selected keywords. All papers which reported expression of homeobox genes in odontogenic lesions were considered.
RESULTS: A total of eleven (11) papers describing expression of homeobox genes in odontogenic lesions were identified. Methods of studies included next generation sequencing, microarray analysis, RT-PCR, Western blotting, in situ hybridization, and immunohistochemistry. The homeobox reported in odontogenic lesions includes LHX8 and DLX3 in odontoma; PITX2, MSX1, MSX2, DLX, DLX2, DLX3, DLX4, DLX5, DLX6, ISL1, OCT4 and HOX C in ameloblastoma; OCT4 in adenomatoid odontogenic tumour; PITX2 and MSX2 in primordial odontogenic tumour; PAX9 and BARX1 in odontogenic keratocyst; PITX2, ZEB1 and MEIS2 in ameloblastic carcinoma while there is absence of DLX2, DLX3 and MSX2 in clear cell odontogenic carcinoma.
CONCLUSIONS: This paper summarized and reviews the possible link between homeobox gene expression in odontogenic lesions. Based on the current available data, there are insufficient evidence to support any definite role of homeobox gene in odontogenic lesions.