目的:与患有口腔恶性肿瘤的患者相比,在Chornobyl事故的清洁工人中建立细胞免疫,咽部的口腔和喉部根据外周血白细胞的亚群组织。
方法:我们检查了112名男性,年龄(56,92±6,17)岁(M±SD),包括26名男性清洁工暴露在10-500mSv的辐射剂量下;20名男性清洁工暴露在504-990mSv的剂量范围内;33名口腔恶性肿瘤患者,咽部的口腔和喉部以及对照组的33名未暴露受试者。使用流式细胞术通过外周血白细胞的差异和活化抗原的表达进行免疫细胞亚群分析。
结果:在评估每组的细胞免疫时,CD19+B淋巴细胞数量减少,CD3+HLA-DR+T-和CD3-HLA-DR+B-淋巴细胞,CD3-16+56+自然杀伤细胞,结合CD8+T淋巴细胞数量的增加。在清理工人(D<500mSv)和癌症患者中,确定了CD4CD8T淋巴细胞相对数量的增加。在清理工人中观察到CD8T淋巴细胞数量的增加和CD4T淋巴细胞数量的减少(D>500mSv),以及癌症患者。此外,单核细胞减少,CD3+16+56+,在肿瘤病理患者中测定CD3+TCRαβ+T淋巴细胞。
结论:获得的结果表明,在切尔诺贝利核电站事故后果的清算参与者和肿瘤病理学研究的患者中,细胞免疫的变化具有单向性,这表明在参与者中持续违反抗肿瘤保护的形成,清算事故的后果是肿瘤发生的基础。确定淋巴细胞数量的变化,单核细胞,粒细胞,CD4+T淋巴细胞,CD4+CD8+未成熟T淋巴细胞,免疫调节比率CD4+/CD8+,CD3+TCRαβ+T淋巴细胞和CD3+16+56+CTL对免疫受损个体的抗肿瘤保护效果和致癌风险的可能性具有额外的影响。
Meta:vyznachhtyzminykitynoidelankyimunnoidesystemyvrotovo损失我很重要。Obstezheno112cholovikivuvitsi(56,92±6,17)rokiv,研究nykh:26uchasnykivlikidatsiínaslidkivaviavariínaChAES,Oproallenykhudiapazonidoz10-500mZvta20uchasnykivlikidatsiidasnaslidkivaviavariínaChAES,Opromalleenykhudiapazonidoz504-990mZv;33khvorykhnazloiakisninovoutvorenniaporozhnynyrota,罗托沃塔·哥坦诺·查斯丁·格洛基三世,IVAiIVV体育场zakhvoriuvannia(grupapozytyvnogokontroliu);ta33osobykontrol\'noígrupy。Analizsubpospopuliatsiofnogorozpodiluprotedlyzaekspressiieiudeterentsytsentoaktyvatsiwithantygenivlekokotsychnotvperyferychnokrovizvykorystanniamprotsytometyvisan.Rezul\'taty.\'kostiCD19+V-limfotsytiv,CD3+HLA-DR+T-iCD3-HLA-DR+V-lifotsytiv,SD3-16+56+天然\'nykhkilerivnafonipidvyshchenniachyslaCD8+T-lifotsyiv。VuchasnykivlidkivavariínaChAES(D<500mZv)taonkologichnokhvorykhpatsiientvvyzenozrostanniavidnosonkostiCD4+T-lifotsyCD8.VuchasnykivlivkidatsiínaslidkivavariínaChAES(D>500mZv),iakivonkologichnokhvorykhpatsiientivspasterigalosiapidvyshchenniavidnosoníkil\'kostiCD8+T-limfotsytiv,znyzhenniakil\'kostiCD4+T-limfotsytiv。Dodatkovovosib,khvorykhnaonkologichnuPatologiuvyznachenoznyzhenniapokaznykivmonotsytiv,SD3+16+56+taSD3+TCRαβ+T-lifotsytiv。Vysnovky.Otrymanirezul\'tatypokazuiut\'odnospriamovanist\'zminkitynnogoimunitetuvuchasnykivlidkividsiidsinaslidkivavariavariínaChAESikhvorykhnadoslidzhenuonkologiu,shchosvidchyt\'proformuvanniavuchasnykivlidkivavariidatsiidasnaslidkivaviavariístikykhporushen\'protypukhlynnogozakhstupidchuntiaonkogenezu.Vyznachenizminypokaznykivlimfotsytiv,monotsytiv,肉芽肿,CD4+T-lifotsytiv,CD4+CD8+nezrilykhT-lifotsytiv,pokaznykaimunoreguliatornogokoefitsiientaCD4+/CD8+,SD3+TCRαβ+T-lifotsytivtaSD3+16+56
OBJECTIVE: to establish cellular immunity in clean-up workers of the
Chornobyl accident compared to patients with malignant neoplasms of the oral cavity, oral and laryngeal parts of the pharynx according to the subpopulation organization of peripheral blood leukocytes.
METHODS: We examined 112 males, age (56,92 ± 6,17) years (M ± SD), including 26 male clean-up workers exposed at the radiation dose 10-500 mSv; 20 male clean-up workers exposed at the dose range 504-990mSv; 33 patients with malignant neoplasms of the oral cavity, oral and laryngeal parts of the pharynx and 33 non-exposed subjects of the control group. Immune cell subsets analysis was performed by the expression of differential and activation antigens of peripheral blood leukocytes using flow cytometry.
RESULTS: In assessing each group\'s cellular immunity, there was a decrease in the number of CD19+ B-lymphocytes, CD3+ HLA-DR+ T- and CD3- HLA-DR+ B-lymphocytes, CD3- 16+ 56+ natural killer cells, combined with an increase in the number of CD8+ T-lymphocytes. An increase in the relative number of CD4+ CD8+ T-lymphocytes was determined in clean-up workers (D < 500 mSv) and cancer patients. An increase in the number of CD8+ T-lymphocytes and a decrease in the number of CD4+ T-lymphocytes were observed in clean-up workers (D > 500 mSv), as well as in cancer patients. In addition, a decrease of monocytes, CD3+ 16+ 56+ , and CD3+ TCRαβ+ T-lymphocytes was determined in patients with oncological pathology.
CONCLUSIONS: The obtained results show the unidirectionality of changes in cellular immunity in the participants of the liquidation of the consequences of the accident at the
Chornobyl nuclear power plant and patients with the investigated oncological pathology, which indicates the formation of persistent violations of antitumor protection in the participants of the liquidation of the consequences of the accident as the basis of oncogenesis. Determining changes in the number of lymphocytes, monocytes, granulocytes, CD4+ T-lymphocytes, CD4+ CD8+ immature T-lymphocytes, the immunoregulatory ratio CD4+ / CD8+, CD3+TCRαβ+ T-lymphocytes and CD3+ 16+ 56+ CTL can have an additional effect on the effectiveness antitumor protection and the probability of oncogenesis risk in immunocompromised individuals.
Meta: vyznachyty zminy klitynnoï lanky imunnoï systemy v uchasnykiv likvidatsiï naslidkiv avariï na ChAES u porivnianni z khvorymy na zloiakisni novoutvorennia porozhnyny rota, rotovoï ta gortannoï chastyn glotky za subpopuliatsiĭnoiu organizatsiieiu leĭkotsytiv peryferychnoï krovi.Materialy i metody. Obstezheno 112 cholovikiv u vitsi (56,92 ± 6,17) rokiv, sered nykh: 26 uchasnykiv likvidatsiï naslidkiv avariï na ChAES, oprominenykh u diapazoni doz 10–500 mZv ta 20 uchasnykiv likvidatsiï naslidkiv avariï na ChAES, oprominenykh u diapazoni doz 504–990 mZv; 33 khvorykh na zloiakisni novoutvorennia porozhnyny rota, rotovoï ta gortannoï chastyn glotky III, IVA i IVV stadiĭ zakhvoriuvannia (grupa pozytyvnogo kontroliu); ta 33 osoby kontrol\'noï grupy. Analiz subpopuliatsiĭnogo rozpodilu provodyly za ekspresiieiu dyferentsiĭnykh ta aktyvatsiĭnykh antygeniv leĭkotsytiv peryferychnoï krovi z vykorystanniam protochnoï tsytometriï.Rezul\'taty. V usikh grupakh obstezhennia vyiavleno znyzhennia vidnosnoï kil\'kosti CD19+ V-limfotsytiv, CD3+ HLA-DR+ T- i CD3- HLA-DR+ V-limfotsytiv, SD3- 16+ 56+ natural\'nykh kileriv na foni pidvyshchennia chysla CD8+ T-limfotsytiv.V uchasnykiv likvidatsiï naslidkiv avariï na ChAES (D < 500 mZv) ta onkologichno khvorykh patsiientiv vyznacheno zrostannia vidnosnoï kil\'kosti CD4+CD8+ T-limfotsytiv. V uchasnykiv likvidatsiï naslidkiv avariï na ChAES (D > 500 mZv),iak i v onkologichno khvorykh patsiientiv sposterigalosia pidvyshchennia vidnosnoï kil\'kosti CD8+ T-limfotsytiv, znyzhennia kil\'kosti CD4+ T-limfotsytiv. Dodatkovo v osib, khvorykh na onkologichnu patologiiu vyznacheno znyzhennia pokaznykiv monotsytiv, SD3+ 16+ 56+ ta SD3+ TCRαβ+ T-limfotsytiv.Vysnovky. Otrymani rezul\'taty pokazuiut\' odnospriamovanist\' zmin klitynnogo imunitetu v uchasnykiv likvidatsiï naslidkiv avariï na ChAES i khvorykh na doslidzhenu onkologichnu patologiiu, shcho svidchyt\' pro formuvannia v uchasnykiv likvidatsiï naslidkiv avariï stiĭkykh porushen\' protypukhlynnogo zakhystu iak pidґruntia onkogenezu. Vyznacheni zminy pokaznykiv limfotsytiv, monotsytiv, granulotsytiv, CD4+ T-limfotsytiv, CD4+ CD8+ nezrilykh T-limfotsytiv, pokaznyka imunoreguliatornogo koefitsiienta CD4+ / CD8+, SD3+ TCRαβ+ T-limfotsytiv ta SD3+ 16+ 56+ tsytotoksychnykh T-limfotsytiv mozhut\' chynyty dodatkovyĭ vplyv na efektyvnist\' protypukhlynnogo zakhystu ta ĭmovirnist\' ryzyku onkogenezu v imunoskomprometovanykh osib.