■睡眠剥夺非常普遍,是由夜班工作或阻塞性睡眠呼吸暂停等疾病引起的。睡眠受损会影响心血管-,免疫-,和神经元系统。最近,我们发表了模拟夜班后的人类血清蛋白质组变化。这项初步的蛋白质组学研究旨在进一步探索夜间睡眠剥夺6小时后人类血清的变化。
■使用OrbitrapEclipse质谱(MS-MS)和高压液相色谱法分析了来自八名自称为健康女性的人血清样品。我们使用了参与者内部设计,其中样本是在晚上睡眠6小时后和第二天晚上睡眠剥夺6小时后采集的。系统生物数据库和生物信息学软件用于分析数据,并与其他已发表的睡眠相关蛋白质组数据集进行比较分析。
■在494种蛋白质中,睡眠剥夺6小时后,发现66个差异表达蛋白(DEP)。功能富集分析揭示了这些DEP与细胞过程如血小板脱颗粒和血液凝固调节改变相关的几种生物学功能的关联。以及与不同的精选基因集的关联。
■这项研究显示了睡眠剥夺6小时后的血清蛋白质组变化,支持先前的发现,表明短暂的睡眠不足会影响几个生物过程,并揭示了与病理状况有关的蛋白质的分子特征,例如凝血和血小板功能的改变,受损的脂质和免疫功能,和细胞增殖。数据可通过ProteomeXchange获得,标识符为PXD045729。这篇论文是睡眠的遗传和其他分子基础的一部分,睡眠障碍,和昼夜节律,包括翻译方法集合。
UNASSIGNED: Sleep deprivation is highly prevalent and caused by conditions such as night shift work or illnesses like obstructive sleep apnea. Compromised sleep affects cardiovascular-, immune-, and neuronal systems. Recently, we published human serum proteome changes after a simulated night shift. This pilot proteomic study aimed to further explore changes in human blood serum after 6 hours of sleep deprivation at night.
UNASSIGNED: Human blood serum samples from eight self-declared healthy females were analyzed using Orbitrap Eclipse mass spectrometry (MS-MS) and high-pressure liquid chromatography. We used a within-participant design, in which the samples were taken after 6 hours of sleep at night and after 6 hours of sleep deprivation the following night. Systems biological databases and bioinformatic software were used to analyze the data and comparative analysis were done with other published sleep-related proteomic datasets.
UNASSIGNED: Out of 494 proteins, 66 were found to be differentially expressed proteins (DEPs) after 6 hours of sleep deprivation. Functional enrichment analysis revealed the associations of these DEPs with several biological functions related to the altered regulation of cellular processes such as platelet degranulation and blood coagulation, as well as associations with different curated gene sets.
UNASSIGNED: This study presents serum proteomic changes after 6 hours of sleep deprivation, supports previous findings showing that short sleep deprivation affects several biological processes, and reveals a molecular signature of proteins related to pathological conditions such as altered coagulation and platelet function, impaired lipid and immune function, and cell proliferation. Data are available via ProteomeXchange with identifier PXD045729. This paper is part of the Genetic and other molecular underpinnings of sleep, sleep disorders, and circadian rhythms including translational approaches Collection.