背景:蜂窝织炎是医院医疗紧急情况中遇到的常见皮肤病。它可以使用抗生素治疗的组合;然而,据报道,病原体金黄色葡萄球菌对目前使用的抗生素产生耐药性。因此,寻找更多的替代草药来源的抗菌药物是至关重要的。
目的:在本研究中,决明子全株的浸渍和索氏提取。(叶子,根,和茎)使用四种极性不同的溶剂进行,即正己烷,乙酸乙酯,乙醇和蒸馏水。粗提物采用琼脂扩散法筛选,比色肉汤微量稀释,针对金黄色葡萄球菌的网格培养和细菌生长曲线分析。使用气相色谱-质谱法(GC-MS)鉴定粗提取物中的植物化学物质。
结果:琼脂孔扩散分析显示,使用乙酸乙酯的提取显示出最大的抑制区,平均直径为15.30mm(根索氏提取物),其次是14.70mm(叶索氏提取物)和13.70mm(根浸渍提取物)。使用乙酸乙酯的根索氏提取物中的最低最低抑菌和最低杀菌浓度分别为0.313和0.625µgµL-1。我们的研究证明,植物的粗提物抑制了金黄色葡萄球菌的生长,这是从浓度增加后6小时滞后期的显着回归延伸(p<0.06,p=0.00003)证明的。基于GC-MS分析,88种植物化学物质由脂肪酸组成,酯类,烷烃,酚类物质,脂肪醇,鉴定了可能有助于抗菌特性的倍半萜和大环化合物,其中32种以前的特征是抗菌,抗氧化剂,和抗炎活性。
结论:乙酸乙酯粗提物优于其他研究溶剂。在这项研究中,C.alata的根和茎显示出对金黄色葡萄球菌的显着抗微生物功效。应深入研究植物88种植物化学物质中的其余56种,以用于更多的药用。
BACKGROUND: Cellulitis is a common skin disease encountered in medical emergencies in hospitals. It can be treated using a combination of antibiotics therapy; however, the causative agent Staphylococcus aureus has been reported to develop resistance towards the currently used antibiotics. Therefore, the search for more alternative herbal origin antimicrobial agents is critical.
OBJECTIVE: In this study, maceration and Soxhlet extraction of the whole plant of Cassia alata Linn. (leaves, roots, and stem) were performed using four solvents with different polarities, namely n-hexane, ethyl acetate, ethanol and distilled water. The crude extracts were screened using agar well diffusion, colorimetric broth microdilution, grid culture and bacterial growth curve analysis against Staphylococcus aureus. The phytochemicals in the crude extracts were identified using Gas Chromatography-Mass Spectrometry (GC-MS).
RESULTS: Agar-well diffusion analysis revealed that extraction using ethyl acetate showed the largest inhibition zone with an average diameter of 15.30 mm (root Soxhlet extract) followed by 14.70 mm (leaf Soxhlet extract) and 13.70 mm (root maceration extract). The lowest minimum inhibitory and minimum bactericidal concentration in root Soxhlet extract using ethyl acetate was 0.313 and 0.625 µg µL-1, respectively. Our study proved that crude extract of the plant suppressed the growth of S. aureus as evidenced from a significant regression extension (p < 0.06, p = 0.00003) of lag phase for 6 h after the treatment with increased concentration. Based on the GC-MS analysis, 88 phytochemicals consist of fatty acids, esters, alkanes, phenols, fatty alcohols, sesquiterpenoids and macrocycle that possibly contributed to the antimicrobial properties were identified, 32 of which were previously characterized for their antimicrobial, antioxidant, and anti-inflammatory activities.
CONCLUSIONS: Ethyl acetate crude extract was better than the other investigated solvents. The root and stem of C. alata showed significant antimicrobial efficacy against S. aureus in this study. The remaining 56 out of 88 phytochemicals of the plant should be intensively studied for more medicinal uses.