美洲念珠(波尔。exPursh)G.L.Nesom&G.I.Baird)在传统医学中用于治疗各种疾病;然而,对这种植物精油的免疫调节活性知之甚少。因此,我们从毛黄的地上部分分离出精油,并评估其化学成分和生物活性。毛黄精油的成分分析表明,主要成分(>2%)是γ-十内酯(13.3%),隐音(9.4%),松油烯-4-醇(9.3%),(E)-肉桂酸甲酯(6.0%),T-cadinol(4.7%),spathulenol(3.6%),8Z-2,3-二氢母霉酯(3.1%),β-水芹烯(3.0%),p-cymen-8-ol(2.2%),3-乙氧基-2-环辛烯-1-酮(2.2%),和反式-对烯-2-烯-1-醇(2.1%)。独特的特征是内酯(高达15%)和聚乙炔(高达3.1%),包括(2Z,8Z)-基质酯和8Z-2,3-二氢基质酯。与其他报告的E.nauseosa精油样品的比较表明,我们的样品与该国其他地区收集的样品不同;然而,他们确实与犹他州中北部收集的样本最相似。药理学研究表明,毛黄精油激活人中性粒细胞Ca2+内流,使这些细胞对随后的激动剂诱导的功能反应脱敏。根据我们之前报道的数据,橙花醇,β-pine烯,spathulenol,Sabinene,γ-萜品烯在人嗜中性粒细胞中有活性,这些化合物是最可能有助于这种免疫调节活性的成分。然而,相对高量的聚乙炔也可能有贡献,因为这些化合物已被表征为有效的免疫调节剂。
Ericameria nauseosa (Pall. ex Pursh) G.L. Nesom & G.I. Baird) is used in traditional medicine to treat various diseases; however, little is known about the immunomodulatory activity of essential oil from this plant. Thus, we isolated essential oil from the aerial parts of E. nauseosa and evaluated their chemical composition and biological activity. Compositional analysis of E. nauseosa essential oil revealed that the main (>2%) components were γ-decalactone (13.3%), cryptone (9.4%), terpinen-4-ol (9.3%), (E)-methyl cinnamate (6.0%), T-cadinol (4.7%), spathulenol (3.6%), 8Z-2,3-dihydromatricaria ester (3.1%), β-phellandrene (3.0%), p-cymen-8-ol (2.2%), 3-ethoxy-2-cycloocten-1-one (2.2%), and trans-p-menth-2-en-1-ol (2.1%). Distinctive features were the lactones (up to 15%) and polyacetylenes (up to 3.1%), including (2Z,8Z)-matricaria ester and 8Z-2,3-dihydromatricaria ester. A comparison with other reported E. nauseosa essential oil samples showed that our samples were distinct from those collected in other areas of the country; however, they did have the most similarity to one sample collected in North Central Utah. Pharmacological studies showed that E. nauseosa essential oil activated human neutrophil Ca2+ influx, which desensitized these cells to subsequent agonist-induced functional responses. Based on our previously reported data that nerolidol, β-pinene, spathulenol, sabinene, and γ-terpinene were active in human neutrophils, these compounds are the most likely constituents contributing to this immunomodulatory activity. However, the relatively high amount of polyacetylenes may also contribute, as these compounds have been characterized as potent immunomodulators.