巴西橡胶树(橡胶树)中的乳胶流,天然橡胶的唯一商业来源(顺式-1,4-聚异戊二烯,NR),使其独特地适合于植物胁迫反应的研究。钙调磷酸酶B样相互作用蛋白激酶(CIPK)作为钙传感器蛋白激酶与钙调磷酸酶B样蛋白(CBL)反应,在植物发育过程中的激素信号转导和对非生物胁迫的响应中起关键作用。然而,人们对它们在橡胶树中的功能知之甚少。在这项研究中,从橡胶树基因组中鉴定出总共12个CBL(HbCBL)和30个CIPK(HbCIPK)基因。结构和系统发育分析将这些CIPK分配到五个组,CBL分配到四个组,并映射到18条橡胶树染色体中的14条。RNA-seq和qPCR分析显示HbCBL和HbCIPK基因在检测的7个橡胶树组织中表达不同,即,乳胶(橡胶生产乳胶的细胞质),吠叫,leaf,根,种子,雌花,和雄花。在叶片发育过程中,两个HbCBL和16个HbCIPK基因的表达呈上升趋势。在乙烯产率刺激和乳胶攻丝处理之后,这两种做法都引起了压力,大多数乳胶表达基因的表达水平显著改变.酵母双杂交试验揭示了HbCBL和HbCIPK的多种组合与乳胶或其他三叶橡胶树组织中大量基因表达的相互作用。然而,所检查的所有HbCBL-HbCIPK复合物均未招募HbSOS1或AtSOS1在酵母细胞中形成功能性耐盐SOS途径.一起来看,结果表明,橡胶树CBL-CIPK网络作为生长中几种不同信号通路的收敛点,发展,以及与乳胶生产有关的应激反应。
Latex flow in Hevea brasiliensis (the Para rubber tree), the sole commercial source of natural rubber (cis-1,4-polyisoprene, NR), renders it uniquely suited for the study of plant stress responses. Calcineurin B-like interacting protein kinases (CIPK) serving as calcium-sensor protein kinases react with calcineurin B-like proteins (CBL) to play crucial roles in hormone signaling transduction and response to abiotic stress in plant developmental processes. However, little is known about their functions in Hevea. In this study, a total of twelve CBL (HbCBL) and thirty CIPK (HbCIPK) genes were identified from the Hevea genome. Structure and phylogenetic analysis assigned these CIPKs to five groups and CBLs to four groups, and mapped onto fourteen of the eighteen Hevea chromosomes. RNA-seq and qPCR analysis showed that the expressions of HbCBL and HbCIPK genes varied in the seven Hevea tissues examined, i.e., latex (cytoplasm of rubber-producing laticifers), bark, leaf, root, seed, female flower, and male flower. The expressions of two HbCBL and sixteen HbCIPK genes showed upward trends during leaf development. Following ethylene yield stimulation and the latex tapping treatment, both practices invoking stress, the expression levels of most latex-expressed genes were significantly altered. Yeast two-hybrid test revealed interactions for multiple combinations of HbCBLs and HbCIPKs with substantial gene expression in latex or other Hevea tissues. However, all the HbCBL-HbCIPK complexes examined did not recruit HbSOS1 or AtSOS1 to form functional salt tolerance SOS pathway in yeast cells. Taken together, the results suggested a role of the Hevea CBL-CIPK network as a point of convergence for several different signaling pathways in growth, development, and stress responses in relation to latex production.