Abstract：
CONCLUSIONS: The putative myristoylome of moss P. patens opens an avenue for studying myristoylation substrates in non-canonical model plants. A myristoylation signal was shown sufficient for membrane targeting and useful for membrane dynamics visualization during cell growth. N-myristoylation (MYR) is one form of lipid modification catalyzed by N-myristoyltransferase that enables protein-membrane association. MYR is highly conserved in all eukaryotes. However, the study of MYR is limited to a few models such as yeasts, humans, and Arabidopsis. Here, using prediction tools, we report the characterization of the putative myristoylome of the moss Physcomitrium patens. We show that basal land plants display a similar signature of MYR to Arabidopsis and may have organism-specific substrates. Phylogenetically, MYR signals have mostly co-evolved with protein function but also exhibit variability in an organism-specific manner. We also demonstrate that the MYR motif of a moss brassinosteroid-signaling kinase is an efficient plasma membrane targeting signal and labels lipid-rich domains in tip-growing cells. Our results provide insights into the myristoylome in a basal land plant and lay the foundation for future studies on MYR and its roles in plant evolution.
摘要：
结论：苔藓P.patens的推定肉豆蔻基因组为研究非规范模型植物中的肉豆蔻酰化底物开辟了途径。肉豆蔻酰化信号显示出足以用于膜靶向并且可用于细胞生长期间的膜动力学可视化。N-肉豆蔻酰化（MYR）是一种由N-肉豆蔻酰基转移酶催化的脂质修饰形式，可实现蛋白质-膜缔合。MYR在所有真核生物中高度保守。然而,MYR的研究仅限于一些模型，如酵母，人类,和拟南芥。这里,使用预测工具，我们报告了苔藓的推定肉豆蔻的特征。我们表明，陆基植物表现出与拟南芥相似的MYR特征，并且可能具有生物特异性底物。系统发育,MYR信号大多与蛋白质功能共同进化，但也以生物体特异性方式表现出变异性。我们还证明了苔藓油菜素类固醇信号传导激酶的MYR基序是有效的质膜靶向信号，并在尖端生长的细胞中标记了富含脂质的结构域。我们的结果为基础陆地植物中的肉豆蔻组提供了见解，并为将来研究MYR及其在植物进化中的作用奠定了基础。
