芦荟(AP)由于其丰富的植物化学成分,作为一种药用植物在各种生物应用中获得了相当大的兴趣。然而,利用纳米技术可以增强AP的治疗益处.此外,阳离子固体脂质纳米粒(CSLNs)具有显著的特性,可以极大地丰富多种生物用途。使用优化方法来实现高质量的CSLN,以最大化AP的治疗效果。因此,采用析因设计研究各种变量对CSLN质量属性的影响.在这项研究中,调查的因素是comprititol888ATO(C-888,X1),泊洛沙姆188(PL188,X2),和壳聚糖(CS,X3),作为独立变量。作为因变量测量的参数包括粒度(Y1),zeta电位(Y2),和包封效率EE(Y3)。通过方差分析(ANOVA)和响应面图确定这些变量之间的关系。结果显示,PL188在减小CSLNS的粒度(范围从207至261nm,1%PL188至167-229nm,3%PL188)中发挥显著作用。相反,CS浓度的增加导致粒径的增加。正ζ电位值的大小取决于CS浓度的增加。此外,较高含量的C-888和PL188将CSLN的EE%从42%提高到86%。此外,观察到优化的AP-CSLN的浓度依赖性抗氧化作用。优化的AP-CSLN在100μg/mL时的抗氧化活性为75%,而AP-SLN和AP溶液的抗氧化活性为62%和60%,分别。抗微生物剂也观察到了类似的改善模式,和优化的AP-CSLN的抗癌活性。这些发现证明了AP-CSLN作为载体系统的潜力,增强AP的生物活性,开辟草药的新可能性。
Aloe perryi (AP) has gained considerable interest as a medicinal herb in various biological applications due to its rich phytochemical composition. However, the therapeutic benefits of AP could be potentiated by utilizing nanotechnology. Moreover, cationic solid lipid nanoparticles (CSLNs) possess remarkable characteristics that can greatly enrich a variety of biological uses. An optimization approach was used to achieve high-quality CSLNs to maximize the therapeutic efficacy of AP. Therefore, a factorial design was used to investigate the influence of various variables on the attributes of CSLNs quality. In this study, the factors under investigation were compritol 888 ATO (C-888, X1), poloxamer 188 (PL188, X2), and chitosan (
CS, X3), which served as independent variables. The parameters measured as dependent variables included particle size (Y1), zeta potential (Y2), and encapsulation efficiency EE (Y3). The relationship among these variables was determined by Analysis of Variance (ANOVA) and response surface plots. The results revealed that PL188 played a significant role in reducing the particle size of CSLNS (ranging from 207 to 261 nm with 1 % PL188 to 167-229 nm with 3 % PL188). Conversely, an increase in the concentration of
CS led to a rise in the particle size. The magnitude of positive zeta potential values was dependent on the increased concentration of
CS. Moreover, the higher amounts of C-888 and PL188 improved the EE% of the CSLNs from 42 % to 86 %. Furthermore, a concentration-dependent antioxidant effect of the optimized AP-CSLNs was observed. The antioxidant activity of the optimized AP-CSLNs at 100 μg/mL was 75 % compared to 62 % and 60 % for AP-SLNs and AP solution, respectively. A similar pattern of improvement was also observed with antimicrobial, and anticancer activities of the optimized AP-CSLNs. These findings demonstrated the potential of AP-CSLNs as a carrier system, enhancing the biological activities of AP, opening new possibilities in herbal medicines.