侵袭性念珠菌病(IC)是一种值得注意的医疗保健相关真菌感染,以高发病率为特征,死亡率,和大量的治疗费用。在这种情况下,白色念珠菌是主要病原体。最近的学术进步揭示了外泌体在关键生物过程中的关键作用,如免疫应答和抗原呈递。这个新兴的研究机构强调了外泌体在医学诊断和治疗领域的潜力,特别是与IC等真菌感染有关。探索IC病理生理学中的外泌体功能不仅增强了我们对疾病的理解,而且为创新的治疗干预措施开辟了新途径。在这次调查中,我们专注于巨噬细胞分泌的外泌体(Exos),未感染和感染白色念珠菌的。我们的目标是提取和分析这些外来体,深入研究其蛋白质组成和亚组的细微差别。为了实现这一点,我们采用了一种称为邻近条形码测定(PBA)的创新技术。这种方法对于我们寻找新的生物靶标至关重要,这可以显着增强白色念珠菌感染的诊断和治疗方法。来自这两种不同细胞状态的外泌体含量的比较分析有望产生有见地的数据,可能导致在理解和治疗这种侵袭性真菌感染方面取得突破。在我们的研究中,我们分析了来自巨噬细胞和白色念珠菌感染的巨噬细胞的外泌体中差异表达的蛋白质,关注ACE2、CD36、CAV1、LAMP2、CD27和MPO等蛋白质。我们还检查了外泌体亚群,在最普遍的亚组中发现MPO的显性表达,和CD36在簇中的不同表达14。这些发现对于理解宿主对白色念珠菌的反应至关重要,并可能为靶向诊断和治疗方法提供信息。我们的研究使我们推断MPO和CD36蛋白可能在白色念珠菌的免疫逃逸机制中起作用。此外,CD36外泌体亚群,通过我们的分析确定,可以作为白色念珠菌感染的潜在生物标志物和治疗靶标。这一见解为了解感染的病理和开发靶向治疗开辟了新的途径。
Invasive candidiasis (IC) is a notable healthcare-associated fungal infection, characterized by high morbidity, mortality, and substantial treatment costs. Candida albicans emerges as a principal pathogen in this context. Recent academic advancements have shed light on the critical role of exosomes in key biological processes, such as immune responses and antigen presentation. This burgeoning body of research underscores the potential of exosomes in the realm of medical diagnostics and therapeutics, particularly in relation to fungal infections like IC. The exploration of exosomal functions in the pathophysiology of IC not only enhances our understanding of the disease but also opens new avenues for innovative therapeutic interventions. In this investigation, we focus on exosomes (Exos) secreted by macrophages, both uninfected and those infected with C. albicans. Our objective is to extract and analyze these exosomes, delving into the nuances of their protein compositions and subgroups. To achieve this, we employ an innovative technique known as Proximity Barcoding Assay (PBA). This methodology is pivotal in our quest to identify novel biological targets, which could significantly enhance the diagnostic and therapeutic approaches for C. albicans infection. The comparative analysis of exosomal contents from these two distinct cellular states promises to yield insightful data, potentially leading to breakthroughs in understanding and treating this invasive fungal infection. In our study, we analyzed differentially expressed proteins in exosomes from macrophages and C. albicans -infected macrophages, focusing on proteins such as ACE2,
CD36, CAV1, LAMP2, CD27, and MPO. We also examined exosome subpopulations, finding a dominant expression of MPO in the most prevalent subgroup, and a distinct expression of
CD36 in cluster14. These findings are crucial for understanding the host response to C. albicans and may inform targeted diagnostic and therapeutic approaches. Our study leads us to infer that MPO and
CD36 proteins may play roles in the immune escape mechanisms of C. albicans. Additionally, the
CD36 exosome subpopulations, identified through our analysis, could serve as potential biomarkers and therapeutic targets for C. albicans infection. This insight opens new avenues for understanding the infection\'s pathology and developing targeted treatments.