■凝集素是碳水化合物结合蛋白结构域。C型凝集素指定了结合钙的要求。蛋白质含有具有多种功能的C型凝集素结构域,包括细胞-细胞粘附,对病原体的免疫反应,和凋亡。本研究旨在研究越南中部白腿虾(凡纳滨对虾)的LvCTL编码基因的特征。
■克隆并测序两个PCR产物(LvCTL3和LvCTL4)。使用生物信息学工具预测LvCTL蛋白的结构和表征。
■结果表明,LvCTL3基因长度为444个核苷酸,与已发布的LvCTL3基因相似98.87%(登录号:KF156943)。多肽序列有147个氨基酸,与参考序列(AGV68681)和LvCTL4基因的长度为417个核苷酸,与已发表的基因(KM387560)相比,同源性为99.52%。推导的多肽序列有138个氨基酸,并且与参考序列(AKA64754)100%相似。LvCTL3的分子量为16.91kDa,等电点(pI)为4.66,而LvCTL4的分子量为15.75和4.58kDa,分别。结构预测结果表明,LvCTL3和LvCTL4具有一个结构域(CTLD),LvCTL3有两个α螺旋和九个β折叠,LvCTL4具有两个α螺旋和八个β折叠。
■我们的结果为C型凝集素的异源表达和生物合成生产提供了必要的信息。
UNASSIGNED: Lectins are carbohydrate-binding protein domains. The C-type lectin designates a requirement for calcium for binding. Proteins contain C-type lectin domains that have a diverse range of functions, including cell-cell adhesion, immune response to pathogens, and apoptosis. This study aimed to investigate the characters of LvCTL-encoding genes from white-leg shrimp ( Litopenaeus vannamei) in Central Vietnam.
UNASSIGNED: Two PCR products (LvCTL3 and LvCTL4) were cloned and sequenced. The structure and characterization of LvCTL proteins were predicted using bioinformatics tools.
UNASSIGNED: The results showed that the LvCTL3 gene was 444 nucleotides in length and 98.87% similar to the published LvCTL3 gene (accession number: KF156943). The polypeptide sequence had 147 amino acids, which were 97.28% identical to the reference sequence (AGV68681) and the LvCTL4 gene had a length of 417 nucleotides and homology of 99.52% compared to the published gene (KM387560). The deduced polypeptide sequence had 138 amino acids, and was 100% similar to the reference sequence (AKA64754). The LvCTL3 had a molecular weight of 16.91 kDa and an isoelectric point (pI) of 4.66, while LvCTL4 had 15.75 and 4.58 kDa, respectively. The structure prediction results showed that LvCTL3 and LvCTL4 had one domain (CTLD), LvCTL3 had two α helices and nine β sheets, and LvCTL4 had two α helices and eight β sheets.
UNASSIGNED: Our results provide essential information for the heterologous expression and biosynthesis production of C-type lectins.