■不断增长的数据表明,肠道微生物组可能导致高脂血症发病率上升。枣醋降低血脂,保护肝脏,并降低氧化能力,然而,目前尚不清楚这是否是由于肠道菌群。为了进一步研究肠道菌群在大枣醋治疗高脂血症中的作用,我们研究了枣醋的作用是否与肠道微生物组的调节有关。
■使用30只雄性ICR小鼠。对照组(CON),高脂饮食(HFD)组,醋组(VIN)每组由10只雌性ICR小鼠连续喂食8周。对于每一种治疗,我们记录了身体质量,肝脏指数,血脂水平,和氧化应激状态。我们还使用高通量16srRNA测序分析了小鼠粪便,以研究大枣醋的降血脂作用与抗氧化活性之间的关系以及它如何影响肠道微生物组。
■枣醋使体重减少了19.92%,血清TC,TG,LDL-C下降25.09%,26.83%,和11.66%,HDL-C增加了1.44倍,血清AST和ALT分别下降26.36%和34.87%,血SOD和GSH-Px分别增加1.35倍和1.60倍,分别。血MDA下降33.21%,肝脏的SOD和GSH-Px增加了1.32倍和1.60倍,分别,HFD小鼠肝脏MDA降低48.96%。肠道微生物组分析表明,枣醋使肠道微生物ASV计数增加了13.46%,高脂饮食小鼠的F/B(Firmicutes/Bacteroidota)比率为2.08倍,该比例与TC呈显著负相关,TG,LDL-C与HDL-C呈正相关醋组中的生物标记细菌包括乳杆菌和乳杆菌,与HDL-C密切相关,SOD,GSH-Px和LDL-C呈阴性,TC,TG。枣醋增加了有氧运动的丰度,包含移动元素,兼性有氧运动的2.84倍,1.45倍,和2.40倍,而潜在病原体的丰度下降了44.72%,根据BugBase的研究。KEGG分析表明,大枣醋主要体现在基因功能的生物学过程中,包括胰高血糖素信号系统,HIF-1信号通路,脂肪细胞因子信号通路,氨基糖,和核苷酸糖代谢,等等。
■基于这些发现,红枣醋可能通过控制肠道微生物组和增强抗氧化能力来降低高脂血症。
UNASSIGNED: Growing data indicate that the gut microbiome may contribute to the rising incidence of hyperlipoidemia. Jujube vinegar lowers lipids, protects the liver, and reduces oxidant capacity, however, it is unknown whether this is due to the gut flora. To further research the role of the gut microbiome in treating hyperlipidemia with jujube vinegar, we looked into whether the action of jujube vinegar is related to the regulation of the gut microbiome.
UNASSIGNED: Thirty male ICR mice were used. The control group (CON), the high-fat diet (HFD) group, and the vinegar group (VIN) each consisted of ten female ICR mice fed consistently for eight weeks. For each treatment, we kept track of body mass, liver index, blood lipid levels, and oxidative stress state. We also analyzed mouse feces using high-throughput 16srRNA sequencing to examine the relationship between jujube vinegar\'s hypolipidemic effect and antioxidant activity and how it affects the gut microbiome.
UNASSIGNED: Jujube vinegar reduced body weight by 19.92%, serum TC, TG, and LDL-C by 25.09%, 26.83%, and 11.66%, and increased HDL-C by 1.44 times, serum AST and ALT decreased by 26.36% and 34.87% respectively, the blood levels of SOD and GSH-Px increased 1.35-fold and 1.60-fold, respectively. While blood MDA decreased 33.21%, the liver\'s SOD and GSH-Px increased 1.32-fold and 1.60-fold, respectively, and the liver\'s MDA decreased 48.96% in HFD mice. The gut microbiome analysis revealed that jujube vinegar increased the intestinal microbial ASV count by 13.46%, and the F/B (Firmicutes/Bacteroidota) ratio by 2.08-fold in high-fat diet mice, and the proportion was significantly inversely correlated with TC, TG, and LDL-C and positively correlated with HDL-C. Biomarker bacteria in the vinegar group included Lactobacillaceae and Lactobacillus, which correlated favorably with HDL-C, SOD, and GSH-Px and negatively with LDL-C, TC, and TG. Jujube vinegar increased the abundance of the Aerobic, Contains Mobile Elements, and Facultative Aerobic by 2.84 times, 1.45 times, and 2.40 times, while decreased the abundance of Potential pathogens by 44.72%, according to the
BugBase study. The KEGG analysis showed that jujube vinegar was predominantly reflected in the biological process of gene function and related to signal transduction pathways, including glucagon signaling system, HIF-1 signaling pathway, adipocytokine signaling pathway, amino sugar, and nucleotide sugar metabolism, and so forth.
UNASSIGNED: Based on these findings, jujube vinegar may reduce hyperlipoidemia by controlling the gut microbiome and enhancing antioxidant capacity.