苔藓动物是全世界发现的水生无脊椎动物苔藓动物。苏丹Fredericellasultana是一种淡水苔藓虫,是粘液虫寄生虫最常见的主要宿主,苔藓四肽。然而,这种苔藓动物的基因组资源有限,这阻碍了对宿主-寄生虫相互作用的分子机制的研究。为了更好地理解这些相互作用,需要建立F.sultana的转录组数据集,通过大规模RNA测序进行功能基因组学分析。从在受控实验室条件下培养的F.sultana类动物中提取总RNA。制备cDNA文库并通过Illumina配对末端测序进行分析。测序数据用于从头转录组组装和功能注释。获得了大约1.18亿个干净的读数,并组装成85,544个重叠群,平均长度为852bp,N50为1,085个基点,和平均GC含量51.4%。使用BLASTX分析注释了总共23,978个(28%)重叠群。在这些成绩单中,4,400个重叠群与腕足动物Lingulaanatina的相似性最高。基于基因本体(GO)注释,得分最高的生物过程类别被归类为细胞过程(27%),代谢过程(24%),和F.sultana转录组的生物学调控(8%)。这项研究首次揭示了F.sultana的转录组,并为该物种提供了全面的遗传资源。我们相信,沙蒙娜的转录组将作为一个有用的基因组数据集,以加速功能基因组学的研究,并将有助于促进全基因组测序和注释。潜在参与生长的候选基因,蛋白水解,并确定了压力/免疫反应,值得进一步调查。
Bryozoans are aquatic invertebrate moss animals that are found worldwide. Fredericella sultana is a freshwater
bryozoan and is the most common primary host of myxozoan parasite, Tetracapsuloides bryosalmonae. However, limited genomic resources are available for this
bryozoan, which hampers investigations into the molecular mechanisms of host-parasite interactions. To better understand these interactions, there is a need to build a transcriptome dataset of F. sultana, for functional genomics analysis by large-scale RNA sequencing. Total RNA was extracted from zooids of F. sultana cultivated under controlled laboratory conditions. cDNA libraries were prepared and were analyzed by the Illumina paired-ends sequencing. The sequencing data were used for de novo transcriptome assembly and functional annotation. Approximately 118 million clean reads were obtained, and assembled into 85,544 contigs with an average length of 852 bp, an N50 of 1,085 bp, and an average GC content 51.4%. A total of 23,978 (28%) contigs were annotated using BLASTX analysis. Of these transcripts, 4,400 contigs had highest similarity to brachiopod species Lingula anatina. Based on Gene ontology (GO) annotation, the most highly scored categories of biological process were categorized into cellular process (27%), metabolic process (24%), and biological regulation (8%) in the transcriptome of F. sultana. This study gives first insights into the transcriptome of F. sultana and provides comprehensive genetic resources for the species. We believe that the transcriptome of F. sultana will serve as a useful genomic dataset to accelerate research of functional genomics and will help facilitate whole genome sequencing and annotation. Candidate genes potentially involved in growth, proteolysis, and stress/immunity-response were identified, and are worthy of further investigation.