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Abstract：
Tetracapsuloides bryosalmonae, a myxozoan endoparasite, causes proliferative kidney disease in salmonids. The life cycle of T. bryosalmonae occurs between invertebrate bryozoan and vertebrate fish hosts. T. bryosalmonae develops in the body cavity of colonial bryozoan and spores are released from mature spore sacs into the water likely through the vestibular pore and infect fish by attaching to their gills. However, very little is known about the transcriptome of this important parasite, which hampers studies into the molecular mechanisms of host-parasite interactions and understanding the parasite biology. In order to circumvent this limitation, we performed de novo transcriptome assembly on the sacs of T. bryosalmonae, collected from infected bryozoan Fredericella sultana. A total of 111.5 million filtered paired-end reads was obtained and assembled into 25,908 contigs corresponding to putative transcripts that were functionally annotated. More than 50% of the assembled transcripts (13,071 contigs) had a significant hit in NCBI non-redundant database. Based on Gene ontology annotation, the most highly scored categories of molecular function of the contigs were related to binding and catalytic activities in T. bryosalmonae. This study provides a global overview of the T. bryosalmonae transcriptome that will be a valuable resource for identifying virulence factors, gene discovery, genome annotation, and vaccine development applications. This data is accessible via NCBI BioProject (PRJNA680464).
摘要：
苔藓四肽，粘液虫体内寄生虫，在鲑鱼中引起增殖性肾脏疾病。苔藓沙门氏菌的生命周期发生在无脊椎动物苔藓虫和脊椎动物鱼类宿主之间。苔藓沙门氏菌在殖民地苔藓虫的体腔中发育，孢子从成熟的孢子囊释放到水中，可能通过前庭孔，并通过附着在鱼的g上感染鱼。然而,对这种重要寄生虫的转录组知之甚少，这阻碍了研究宿主-寄生虫相互作用的分子机制和理解寄生虫生物学。为了规避这一限制，我们在苔藓沙门氏菌的囊上进行了从头转录组组装，从受感染的苔藓虫中收集。总共获得了1.115亿个过滤的配对末端读段，并组装成25,908个重叠群，对应于功能注释的推定转录本。在NCBI非冗余数据库中，超过50％的组装转录本（13,071个重叠群）具有显着的命中。基于基因本体注释,重叠群的分子功能得分最高的类别与苔藓的结合和催化活性有关。本研究提供了苔藓沙门氏菌的转录组的全球概述，这将是鉴定毒力因子的宝贵资源，基因发现,基因组注释，和疫苗开发应用。该数据可通过NCBIBioProject(PRJNA680464)获取。
