大麻是一种众所周知的植物,以其精神作用;然而,它的许多衍生物,如大麻二酚(CBD),包含几种治疗应用。四氢大麻酚(THC)是负责精神活性的主要大麻衍生物,而CBD是非精神药物。出于这个原因,CBD在过去十年中得到了更多的利用。CBD已与多种抗癌特性有关,当与光动力疗法(PDT)结合使用时,有可能更有效地根除肿瘤。在这项研究中,CBD用于治疗MCF-7乳腺癌细胞,其次是体外PDT联合治疗。传统的乳腺癌治疗方式,如化疗,放射治疗,等。已经报道了诱导许多不良副作用,疾病复发,生活质量低。在这项研究中,细胞暴露于不同浓度的CBD(即,1.25、2.5、5、10和20μg/mL),并在处理后12和24小时孵育。然后将最佳剂量用于联合治疗。形态学和生化分析,包括乳酸脱氢酶(LDH)膜的完整性,三磷酸腺苷(ATP)的活力,和锥虫蓝排除试验的活力,用于检查治疗后的细胞反应。然后在金丝桃素-金纳米颗粒介导的PDT组合中利用最佳浓度。结果显示,以剂量依赖的方式,细胞死亡的常规形态学特征,比如空泡化,咯咯声,在处理的细胞中观察到漂浮。生化反应表明LDH增加,ATP的减少,和生存能力的降低。这项研究表明,CBD在体外培养的MCF-7乳腺癌细胞中诱导细胞死亡。联合治疗的免疫荧光结果表明,细胞死亡是通过凋亡发生的。总之,这项研究表明,CBD和PDT联合治疗可通过诱导凋亡有效杀死MCF-7乳腺癌细胞。
Cannabis sativa is a well-known plant for its psychoactive effects; however, its many derivatives, such as Cannabidiol (CBD), contain several therapeutic applications. Tetrahydrocannabinol (THC) is the main cannabis derivative responsible for psychoactive properties, while CBD is non-psychotropic. For this reason, CBD has been more exploited in the last decade. CBD has been connected to multiple anticancer properties, and when combined with photodynamic therapy (PDT), it is possible to eradicate tumors more effectively. In this study, CBD was utilized to treat MCF-7 breast cancer cells, followed by in vitro PDT combination therapy. Conventional breast cancer treatment modalities such as chemotherapy, radiotherapy, etc. have been reported for inducing a number of undesirable side effects, recurrence of the disease, and low quality of life. In this study, cells were exposed to varying concentrations of CBD (i.e., 1.25, 2.5, 5, 10, and 20 μg/mL) and incubated 12 and 24 h after treatment. The optimal doses were then used in combination therapy. Morphology and biochemical assays, including lactate dehydrogenase (LDH) for membrane integrity, adenosine triphosphate (ATP) for viability, and trypan blue exclusion assay for viability, were used to examine cellular responses after treatments. The optimal concentration was then utilized in Hypericin-Gold nanoparticles mediated PDT combination. The results revealed that, in a dose-dependent manner, conventional morphological characteristics of cell death, such as vacuolization, blebbing, and floating were observed in treated cells. The biochemical responses demonstrated an increase in LDH, a decrease in ATP, and a reduction in viability. This study demonstrated that CBD induces cell death in MCF-7 breast cancer cells cultured in vitro. The immunofluorescence results of combination therapy indicated that cell death occurred via apoptosis. In conclusion, this study proposes that the CBD and PDT combination therapy is effective in killing MCF-7 breast cancer cells in vitro by induction of apoptosis.