Abstract：
In this study, a novel selenium@zeolitic imidazolate framework core/shell nanocomposite stabilised with alginate was used to improve the anti-tumour activity of curcumin. The developed alginate-stabilised curcumin-loaded selenium@zeolitic imidazolate framework (Alg@Cur@Se@ZIF-8) had a mean diameter of 159.6 nm and polydispersity index < 0.25. The release of curcumin from the nanocarrier at pH 5.4 was 2.69 folds as high as at pH 7.4. The bare nanoparticles showed haemolytic activity of about 12.16% at a concentration of 500 µg/mL while covering their surface with alginate reduced this value to 5.2%. By investigating cell viability, it was found that Alg@Cur@Se@ZIF-8 caused more cell death than pure curcumin. Additionally, in vivo studies showed that Alg@Cur@Se@ZIF-8 dramatically reduced tumour growth compared to free curcumin in 4T1 tumour-bearing mice. More importantly, the histological study confirmed that the developed drug delivery system successfully inhibited lung and liver metastasis while causing negligible toxicity in vital organs. Overall, due to the excellent inhibitory activity on cancerous cell lines and tumour-bearing animals, Alg@Cur@Se@ZIF-8 can be considered promising for breast cancer therapy.
摘要：
在这项研究中,用海藻酸盐稳定的新型硒@沸石咪唑酯骨架核/壳纳米复合材料用于提高姜黄素的抗肿瘤活性。开发的藻酸盐稳定的姜黄素负载的硒@沸石咪唑酯骨架（Alg@Cur@Se@ZIF-8）的平均直径为159.6nm，多分散指数<0.25。在pH5.4时,姜黄素从纳米载体的释放是pH7.4时的2.69倍。裸露的纳米颗粒在500μg/mL的浓度下显示出约12.16%的溶血活性,而用海藻酸盐覆盖其表面将该值降至5.2%。通过研究细胞活力，发现Alg@Cur@Se@ZIF-8比纯姜黄素引起更多的细胞死亡。此外,体内研究表明，与4T1荷瘤小鼠中的游离姜黄素相比，Alg@Cur@Se@ZIF-8显着降低了肿瘤生长。更重要的是,组织学研究证实，开发的药物递送系统成功地抑制了肺和肝转移，同时对重要器官的毒性可忽略不计。总的来说,由于对癌细胞系和荷瘤动物具有优异的抑制活性，Alg@Cur@Se@ZIF-8可以被认为是有前途的乳腺癌治疗。
