This study investigates the anti-inflammatory, analgesic, and antioxidant properties of polyphenols extracted from Brassica oleracea var. capitata (cabbage) ethanolic extract (BOE). Given the historical use of cabbage in traditional medicine for treating various ailments, this research aims to validate these effects scientifically. The study involved the characterization of BOE\'s bioactive compounds using Fourier Transform Infrared Spectroscopy (FTIR) and Liquid Chromatography-Diode Array Detection-Electro-Spray Ionization Mass Spectrometry (HPLC-DAD-ESI MS) analysis. We assessed the anti-inflammatory and analgesic effects of topical and oral BOE administration on rodent models with acute and subacute inflammation. Additionally, the antioxidant capacity of orally administered BOE was evaluated. The results showed that BOE possesses significant levels of phenolic compounds with a potent antioxidant activity. The topical administration of BOE demonstrated notable anti-inflammatory effects in the tested rodent models, which were comparable with nonsteroidal anti-inflammatory drugs. These findings suggest that BOE could be a valuable natural remedy for inflammation-related conditions, supporting its traditional uses and highlighting its potential for further pharmacological development.

Glucosinolates (GSLs) are plant secondary metabolites commonly found in the cruciferous vegetables of the Brassicaceae family, offering health benefits to humans and defense against pathogens and pests to plants. In this study, we investigated 23 GSL compounds\' relative abundance in four tissues of five different Brassica oleracea morphotypes. Using the five corresponding high-quality B. oleracea genome assemblies, we identified 183 GSL-related genes and analyzed their expression with mRNA-Seq data. GSL abundance and composition varied strongly, among both tissues and morphotypes, accompanied by different gene expression patterns. Interestingly, broccoli exhibited a nonfunctional AOP2 gene due to a conserved 2OG-FeII_Oxy domain loss, explaining the unique accumulation of two health-promoting GSLs. Additionally, transposable element (TE) insertions were found to affect the gene structure of MAM3 genes. Our findings deepen the understanding of GSL variation and genetic regulation in B. oleracea morphotypes, providing valuable insights for breeding with tailored GSL profiles in these crops.

The spectral composition of some light-emitting diodes (LEDs) reportedly results in higher crop yield, prevents wilting, and reduces thermal damage to plants. The use of LEDs for postharvest storage and shelf-life extension has been limited, but the potential of this technology will allow for greater applications in horticulture and the food industry. In this experiment, \'Winterbor\' kale (Brassica oleracea) and \'Melody\' spinach (Spinacia oleracea) plants were measured for the light compensation point and stomatal response under 14 different wavelengths of light ranging from 405 to 661 nm. Data collected from these measurements were used to select two different wavelengths of LEDs and determine the proper irradiance levels for an LED irradiance storage test on spinach and kale. Treatments comprising blue, red, and amber lights were effective at increasing the stomatal opening, while the green light resulted in reduced stomatal opening. For spinach, the light response curve showed that light compensation points at 500 nm and 560 nm were 65.3 and 64.7 μmol m-2 s-1, respectively. For kale, the light compensation points at 500 nm and 560 nm were 50.8 and 44.1 μmol m-2 s-1, respectively. For the storage test experiment at room temperature, kale and spinach were stored under four different treatments: dark treatment (control), standard white fluorescent light, 500 nm, and 560 nm LED wavelengths. For spinach, the moisture content was 70.1% at 560 nm and 53.7% for dark, moisture losses of 41.5% under the 560-nm treatment and 52.0% for the dark treatment. The fresh basis moisture content was 74.6% at 560 nm and 59.3% in the dark. Moisture loss under the 560 nm treatment was 39.6% while the dark treatment had a 54.0% moisture loss. A visual assessment scale was monitored, 560 nm resulted in the top visual quality for kale compared to the other treatments with the lowest visual quality under the dark treatment at day 4. For spinach, the visual quality for 560 nm treatment was statistically the standard white fluorescent light and 500 nm, with poor-quality product occurring by day 4 and the lowest-quality product occurring at day 5. The LED treatments improved the shelf life of spinach and kale, likely as a result of stomatal aperture closure, photosynthetic rate near the light compensation point and stability of the atmospheric moisture content. This study provides valuable information on the extension of the shelf life of leafy greens during storage. Reducing fresh produce waste in grocery stores will increase revenue, thereby benefiting the Canadian economy while providing social and environmental benefits that entail increased food security and reduced food waste.

This research introduces an extensive dataset of unprocessed aerial RGB images and orthomosaics of Brassica oleracea crops, captured via a DJI Phantom 4. The dataset, publicly accessible, comprises 244 raw RGB images, acquired over six distinct dates in October and November of 2020 as well as 6 orthomosaics from an experimental farm located in Portici, Italy. The images, uniformly distributed across crop spaces, have undergone both manual and automatic annotations, to facilitate the detection, segmentation, and growth modelling of crops. Manual annotations were performed using bounding boxes via the Visual Geometry Group Image Annotator (VIA) and exported in the Common Objects in Context (COCO) segmentation format. The automated annotations were generated using a framework of Grounding DINO + Segment Anything Model (SAM) facilitated by YOLOv8x-seg pretrained weights obtained after training manually annotated images dated 8 October, 21 October, and 29 October 2020. The automated annotations were archived in Pascal Visual Object Classes (PASCAL VOC) format. Seven classes, designated as Row 1 through Row 7, have been identified for crop labelling. Additional attributes such as individual crop ID and the repetitiveness of individual crop specimens are delineated in the Comma Separated Values (CSV) version of the manual annotation. This dataset not only furnishes annotation information but also assists in the refinement of various machine learning models, thereby contributing significantly to the field of smart agriculture. The transparency and reproducibility of the processes are ensured by making the utilized codes accessible. This research marks a significant stride in leveraging technology for vision-based crop growth monitoring.

BACKGROUND: Brassica oleracea var. acephala, commonly referred to as kale, is a well-documented plant species, a food crop but well recognized for its capacity to endure and manage the accumulation of heavy metals. In this research, the phytoremediation potential of kale was evaluated based on cadmium intake, utilizing three distinct kale varieties originating from Bosnia and Herzegovina. All kales were grown in controlled conditions, with different concentrations of cadmium (Cd), a known strong pollutant found in small concentrations in soil under normal environmental conditions. After the root length analysis and cadmium atomic spectrometry, we utilized quantitative PCR (qPCR) and cycle threshold (Ct) values to calculate the expression levels of five genes associated with Cd heavy metal response: Mitogen-activated protein kinase 2 (MAPK2), Farnesylated protein 26 and 27 (HIPP26, HIPP27), Natural resistance-associated macrophage protein 6 (RAMP6), and Heavy metal accumulator 2 (HMA2).
RESULTS: The atomic reader\'s analysis of rising cadmium concentrations revealed a proportional decline in the length of kale roots. The gene expression levels corresponded to cadmium stress differently among varieties, but mostly showing notable up-regulations under Cd stress, indicating the strong Cd presence within the plant.
CONCLUSIONS: This study demonstrated differences in gene expression behavior among three B. oleracea varieties from Bosnia and Herzegovina, indicating and filtering the Cd-resistant kale, and kale varieties suitable for phytoremediation. For the first time, such a study was conducted on kale varieties from Bosnia and Herzegovina, analyzing the impact of cadmium on the growth and resilience of these species.

Negative effects of salt stress may be counteracted by adequate management of sulfated nutrition. Herein, we applied 3.50, 4.25, and 5.00 mM SO42- in a nutrient solution to counteract salt stress induced by 75 and 150 mM NaCl in cabbage cv. Royal. The increase in NaCl concentration from 75 to 150 mM reduced the contents of macronutrients and micronutrients in the shoot. When increasing from 3.50 to 4.25 mM SO42-, the contents of nitrogen (N), phosphorous (P), potassium (K), calcium (Ca), magnesium (Mg), and sulfur (S) in shoots were enhanced, at both concentrations of NaCl. Increasing from 3.50 to 4.25 mM SO42- enhanced iron (Fe), zinc (Zn), manganese (Mn), and sodium (Na) concentrations with 75 mM NaCl. With 150 mM NaCl, the increase from 3.50 to 4.25 mM SO42- enhanced the contents of Cu and Mn, but also those of Na. Chlorophylls a, b, and total decreased as the concentration of SO42- increased in plants treated with 150 mM NaCl. With 75 mM NaCl, carotenoid concentration had a positive relationship with SO42-. Hence, the 4.25 mM SO42- concentration increased the contents of macronutrients and micronutrients in the presence of 75 mM NaCl, while, with 150 mM NaCl, it improved the contents of macronutrients except K. The chlorophyll a/chlorophyll b ratio remained close to 3 when the plants were treated with 5.00 mM SO42-, regardless of NaCl. Similarly, this level of SO42- increased the concentration of carotenoids, which translated into reductions in the total chlorophyll/carotenoid ratios, indicating a protective effect of the photosynthetic apparatus. It is concluded that higher doses of sulfur favor the accumulation of nutrients and increase the concentration of carotenoids under salt stress.

Broccoli is a rich source of diverse bioactive compounds, but how their contents are influenced by different growing seasons and variations in broccoli head sizes remains elusive. To address this question, we quantified sixteen known bioactive compounds and seven minerals in broccoli with varying head sizes obtained in two different growing seasons. Our results suggest that the contents of vitamin C, total phenols, carotenoids, and glucoraphanin were significantly higher in samples from the summer-autumn season, showing increases of 157.46%, 34.74%, 51.80%, and 17.78%, respectively, compared with those from the winter-spring season. Moreover, chlorogenic acid is a phenolic compound with relatively high contents among the six detected, while beta-sitosterol is the sterol with relatively high contents. Further, principal component analysis was conducted to rank the comprehensive scores of the profiles of phenolic compounds, phytosterols, and minerals, demonstrating that the broccoli samples grown during the summer-autumn season achieved the highest composite scores. Our results indicate that broccoli heads from the summer-autumn season are richer in a combination of bioactive compounds and minerals than those from the winter-spring season based on the composite score. This study extends our understanding of the nutrition profiles in broccoli and also lays the foundation for breeding broccoli varieties with improved nutrition quality.

The objective of this work was to gain insight into the operating conditions that affect the efficiency of ultrasound-assisted extraction (UAE) parameters to achieve the best recovery of bioactive compounds from broccoli leaf and floret byproducts. Therefore, total phenolic content (TPC) and the main sulfur bioactive compounds (sulforaphane (SFN) and glucosinolates (GLSs)) were assayed. Distilled water was used as solvent. For each byproduct type, solid/liquid ratio (1:25 and 2:25 g/mL), temperature (25, 40, and 55 °C), and extraction time (2.5, 5, 7.5, 10, 15, and 20 min) were the studied variables to optimize the UAE process by using a kinetic and a cubic regression model. TPC was 12.5-fold higher in broccoli leaves than in florets, while SFN was from 2.5- to 4.5-fold higher in florets regarding the leaf\'s extracts obtained from the same plants, their precursors (GLS) being in similar amounts for both plant tissues. The most efficient extraction conditions were at 25 °C, ratio 2:25, and during 15 or 20 min according to the target phytochemical to extract. In conclusion, the type of plant tissue and used ratio significantly influenced the extraction of bioactive compounds, the most efficient UAE parameters being those with lower energy consumption.

The presence of toxic metals in residential areas near abandoned gold mine tailings is a major environmental issue. This study mainly aimed to investigate the elemental distribution of both toxic and essential elements in soils and leafy vegetables (Brassica oleracea) collected from eight different sites around the Davidsonville residential area, located closer to the abandoned Princess gold mine dump, Johannesburg, South Africa. The nutritional value of vegetables in the human diet was determined to assess their value to their health. The vegetables contained metals in the following descending order: Ca > Mg > Ca > Sb > Pb > Fe > Mo > Cr > Se > As > V > Ni > Co > Cd. The bioaccumulation factor (BAF) revealed that vegetables tend to accumulate most metals even (toxic) during the transfer and translocation process. Based on the recommended daily allowance (%RDA) the vegetables showed to contribute 152%, 84% and 75% toward RDA for Se, V and Ca, respectively for most adults and these play a role in human metabolic activities. The vegetables were found to be a good source of essential elements (Ca, Mg, Ni, Na, Fe) but with some traces of toxic metals such as Pb, As and Sb. Based on the health risk assessment, the vegetable posed an adverse health hazard for human consumption due to metals with high HRI >1.

UNASSIGNED: Globally, hypereutrophic conditions in major water reservoirs used for irrigation purposes, promote the co-existence of cyanotoxins and other pollutants such as linear alkylbenzene sulfonate (LAS). LAS is known to alter the permeability of membranes and promote the uptake of other pollutants by plants. In light of the potential human health risks and prevailing hypereutrophic conditions in some catchments in South Africa, we investigated the combined effects of LAS and microcystins (MCs) on food plants when cyanobacteria infested water is used to irrigate terrestrial crops.
UNASSIGNED: To understand the potential risks, pot-culture experiments were conducted to assess the effect of LAS on the accumulation of MCs in Brassica oleracea (cabbage) and Solanum tuberosum (potato) plants. The plants were watered with dam water containing 3.48 mg L -1 of the LAS (sodium dodecyl sulfate) and MCs (MC-LR: 10.47 ± 3.879; 6.158 ± 4.127 for MC-RR and 8.160 ± 2.544 for MC-YR μg L -1) for 20 days.
UNASSIGNED: The presence of LAS, at environmentally relevant concentrations in the irrigation water, did not enhance the uptake of MCs in the two plants, as demonstrated by statistically insignificant differences in the means of the treatments (with and without LAS). In addition, the presence of LAS, high pH, electrical conductivity (EC), and cyanotoxins in the water did not affect the total chlorophyll or the well-being of the plants. However, in some cases the levels of MCs bioaccumulated by the two plants exceeded the WHO recommended tolerable daily intake (TDI).
UNASSIGNED: These findings imply that the tested levels of LAS and MCs did not have any synergic effects on the two plant species, but irrigating food crops with such water still poses a human health risk.