蝙蝠是各种能影响人类和动物的传染因子的天然宿主,它们在地理上分布广泛。近年来,蝙蝠相关病原体的流行在全球范围内激增,因此对蝙蝠及其外寄生虫产生了极大的兴趣。在这项研究中,我们特别选择了小翅目作为宿主,并在南坚彝族自治县进行了蝙蝠捕获,大理白族自治州,另一个在牟定镇,楚雄彝族自治州,位于云南省,中国。从蝙蝠身体表面精心收集外寄生虫,与血液样本一起进行后续分析。收集之后,对体外寄生虫进行了有条理的鉴定,并进行了全面的生态分析。此外,从蝙蝠血和蝙蝠蝇中提取DNA,使用常规PCR技术对四种病原体进行分子筛选:anap理虫。,巴贝西亚。,肝体sp.,和巴尔通体sp。捕获工作总共产生了37μm。fuliginosus,从中回收了388个外寄生虫,包括197只gamasid螨(Cr=50.77%,PM=94.59%,MA=5.32,MI=5.63)和191只蝙蝠蝇(Cr=49.23%,PM=75.68%,MA=5.16,MI=6.82)。值得注意的是,Steatonyssusnyctali(Y=0.28,m*/m=2.44)和Nycteribiaallotopa(Y=0.23,m*/m=1.54)在不同个体中占主导地位。表现出聚集的分布格局。巴尔通体的感染率。被确定为蝙蝠中的18.92%(7/37)和蝙蝠蝇中的37.17%(71/191),根据37只蝙蝠和191只蝙蝠苍蝇的测试。系统发育分析表明,巴尔通体序列与中国和韩国的蝙蝠和蝙蝠蝇中发现的序列相似。这项研究不仅有助于我们理解夫氏螺旋藻的体外寄生虫感染,而且为潜在探索其作为载体的作用奠定了基础。
Bats serve as natural hosts for various infectious agents that can affect both humans and animals, and they are geographically widespread. In recent years, the prevalence of bat-associated pathogens has surged on a global scale, consequently generating significant interest in bats and their ectoparasites. In this study, we specifically selected the Miniopterus fuliginosus as the host and conducted bat captures in Nanjian Yi Autonomous County, Dali Bai Autonomous Prefecture, and the other in Mouding Township, Chuxiong Yi Autonomous Prefecture, located in Yunnan Province, China. Ectoparasites were meticulously collected from the bat body surface, alongside blood samples for subsequent analyses. Following collection, the ectoparasites were methodically identified and subjected to comprehensive ecological analysis. Additionally, DNA was extracted from both the bat blood and bat flies, with conventional PCR techniques utilized for molecular screening of four pathogens: Anaplasma sp., Babesia sp., Hepatozoon sp., and Bartonella sp. The capture efforts yielded a total of 37 M. fuliginosus, from which 388 ectoparasites were recovered, including 197 gamasid mites (Cr = 50.77%, PM = 94.59%, MA = 5.32, MI = 5.63) and 191 bat flies (Cr = 49.23%, PM = 75.68%, MA = 5.16, MI = 6.82). Notably, Steatonyssus nyctali (Y = 0.28, m*/m = 2.44) and Nycteribia allotopa (Y = 0.23,m*/m = 1.54) predominated among different individuals of M. fuliginosus, exhibiting an aggregated distribution pattern. The infection rates of Bartonella sp. were identified to be 18.92% (7/37) among bats and 37.17% (71/191) among bat flies, based on the testing of 37 bats and 191 bat flies. Phylogenetic analysis demonstrated that the Bartonella sequences exhibited similarity to those found in bats and bat flies within China and South Korea. This study not only contributes to our comprehension of ectoparasite infection in M. fuliginosus but also establishes a foundation for potential exploration of their role as vectors.