拟杆菌和Phocaeicola,拟杆菌科的成员,是最早定植于人类婴儿肠道的微生物之一。虽然已知这些微生物可以从母亲传播给孩子,我们对共享和潜在传播的特定菌株的理解是有限的.在这项研究中,我们旨在调查母亲及其婴儿中的拟杆菌和Phocaeicola的共有菌株。我们分析了从PreventADALL研究中招募的18孕周孕妇的粪便样本,以及早期婴儿的后代样本,包括出生后10分钟内采集的皮肤拭子样本,第一个可用的粪便样本(胎粪),和3个月大的粪便样本。我们筛选了464个胎粪样本的拟杆菌科,随后选择144对母子进行纵向分析,基于拟杆菌科的存在,纵向样本可用性,和交付模式。我们的结果表明,拟杆菌科成员主要在阴道分娩婴儿的样本中检测到。我们发现了高患病率的Phocaeicolavulgatus,Phocaeicoladorei,拟杆菌,和拟杆菌在母亲和阴道出生的婴儿身上。然而,在应变水平,我们观察到只有两种菌株的高流行率:B.caccae菌株和P.vulgatus菌株。值得注意的是,B.caccae菌株被鉴定为母婴共享菌株的新成分,在全世界公开的宏基因组中也观察到其高患病率。我们的研究结果表明,分娩方式可能在塑造婴儿肠道微生物群的早期定植中起作用。特别是拟杆菌科成员的定植。重要性我们的研究提供了证据,表明婴儿出生后10分钟内皮肤上存在拟杆菌科菌株,在胎粪样本中,在3个月大的粪便样本中,阴道分娩的婴儿与母亲分享。使用应变分辨率分析,我们确定了两个菌株,属于拟杆菌属和Phocaeicolavulgatus,在母亲和婴儿之间共享。有趣的是,B.caccae菌株在世界范围内表现出很高的患病率,而P.vulgatus菌株不太常见。我们的发现还表明,阴道分娩与拟杆菌科成员的早期定植有关,而剖宫产分娩与延迟定植有关。鉴于这些微生物可能影响结肠环境,我们的结果表明,在菌株水平上了解细菌与宿主的关系可能对婴儿的健康和以后的发育有影响.
Bacteroides and Phocaeicola, members of the family
Bacteroidaceae, are among the first microbes to colonize the human infant gut. While it is known that these microbes can be transmitted from mother to child, our understanding of the specific strains that are shared and potentially transmitted is limited. In this study, we aimed to investigate the shared strains of Bacteroides and Phocaeicola in mothers and their infants. We analyzed fecal samples from pregnant woman recruited at 18 weeks of gestation from the PreventADALL study, as well as offspring samples from early infancy, including skin swab samples taken within 10 min after birth, the first available fecal sample (meconium), and fecal samples at 3 months of age. We screened 464 meconium samples for
Bacteroidaceae, with subsequent selection of 144 mother-child pairs for longitudinal analysis, based on the presence of
Bacteroidaceae, longitudinal sample availability, and delivery mode. Our results showed that
Bacteroidaceae members were mainly detected in samples from vaginally delivered infants. We identified high prevalences of Phocaeicola vulgatus, Phocaeicola dorei, Bacteroides caccae, and Bacteroides thetaiotaomicron in mothers and vaginally born infants. However, at the strain level, we observed high prevalences of only two strains: a B. caccae strain and a P. vulgatus strain. Notably, the B. caccae strain was identified as a novel component of mother-child shared strains, and its high prevalence was also observed in publicly available metagenomes worldwide. Our findings suggest that mode of delivery may play a role in shaping the early colonization of the infant gut microbiota, in particular the colonization of Bacteroidaceae members. IMPORTANCE Our study provides evidence that
Bacteroidaceae strains present on infants\' skin within 10 min after birth, in meconium samples, and in fecal samples at 3 months of age in vaginally delivered infants are shared with their mothers. Using strain resolution analyses, we identified two strains, belonging to Bacteroides caccae and Phocaeicola vulgatus, as shared between mothers and their infants. Interestingly, the B. caccae strain showed a high prevalence worldwide, while the P. vulgatus strain was less common. Our findings also showed that vaginal delivery was associated with early colonization of Bacteroidaceae members, whereas cesarean section delivery was associated with delayed colonization. Given the potential for these microbes to influence the colonic environment, our results suggest that understanding the bacterial-host relationship at the strain level may have implications for infant health and development later in life.