背景:口腔癌是一种影响大、预后差的恶性肿瘤。Naringenin,柑橘类水果中发现的黄酮类化合物及其抗炎和抗氧化特性提供了潜在的治疗益处。然而,关于柚皮素对人舌癌CAL-27细胞的影响的研究有限。本研究旨在阐明柚皮素与舌癌的相关性,从而确定了针对舌癌的药物干预的潜在治疗候选物。
方法:采用细胞计数试剂盒-8、线粒体膜电位测定JC-1、AnnexinV-FITC凋亡检测等方法研究柚皮素对CAL-27细胞凋亡的影响及其机制。细胞周期,和细胞凋亡分析,活性氧种类测定和蛋白质印迹。
结果:结果表明柚皮素以剂量依赖性方式显著诱导CAL-27细胞凋亡。机械上,柚皮素诱导的细胞凋亡是通过上调Bid和下调Bcl-xl介导的,这导致ROS的产生增加。
结论:研究结果表明,柚皮素可能是通过调节Bid和Bcl-xl信号通路诱导凋亡细胞死亡来治疗口腔癌的有希望的候选者。
BACKGROUND: Oral cancer is a malignant tumor with a high impact and poor prognosis. Naringenin, a flavonoid found in citrus fruits and its anti-inflammatory and antioxidant properties offer potential therapeutic benefits. However, limited studies have been conducted on the impact of naringenin on human tongue carcinoma CAL-27 cells. This study aims to elucidate the correlation between naringenin and tongue cancer, thereby identifying a potential therapeutic candidate for drug intervention against tongue cancer.
METHODS: The effect of naringenin on the apoptosis of CAL-27 cells and its mechanism were studied by cell counting kit-8, mitochondrial membrane potential assay with JC-1, Annexin V-- FITC apoptosis detection, cell cycle, and apoptosis analysis, Reactive Oxygen Species assay and Western blot.
RESULTS: The results showed that naringenin significantly induced apoptosis in CAL-27 cells in a dose-dependent manner. Mechanistically, naringenin-induced apoptosis was mediated through the upregulation of Bid and downregulation of Bcl-xl, which led to increased generation of ROS.
CONCLUSIONS: The findings suggested that naringenin may represent a promising candidate for the treatment of oral cancer by inducing apoptotic cell death via modulation of the Bid and Bcl-xl signaling pathways.