来自大肠杆菌的细菌细胞外囊泡(BEV),菌株A5922被用作治疗结肠癌细胞的治疗工具,HT-29.BEV诱导的氧化应激,并观察到线粒体自噬,被称为线粒体自噬,对开始治疗至关重要。有丝分裂,由HT-29细胞中的BEV诱导,产生的腺癌细胞毒性,并阻止细胞生长。线粒体自噬的触发因素,活性氧产生的增加导致细胞氧化应激,最终导致细胞死亡。线粒体膜电位降低,PINK1表达的增加证实了氧化应激的参与。BEV触发了细胞毒性,和HT-29类癌细胞中的线粒体自噬,通过Akt/mTOR途径连接细胞氧化应激,有效地发挥了导致细胞死亡的作用。这些发现证实了BEV作为一种合理的治疗工具的潜力,并可能预防结直肠癌。
Bacterial-extracellular-vesicles (
BEVs) derived from Escherichia coli, strain-A5922, were used as a therapeutic tool to treat colon cancer cells, HT-29.
BEVs induced oxidative stress, and observed mitochondrial autophagy, known as mitophagy, were crucial in initiation of treatment. The mitophagy, induced by the
BEVs in HT-29 cells, produced adenocarcinomic cytotoxicity, and stopped the cells growth. The trigger for mitophagy, and an increase in productions of reactive oxygen species led to cellular oxidative stress, that eventually led to cells death. A reduction in the mitochondrial membrane potential, and an increase in the PINK1 expressions confirmed the oxidative stress involvements. The
BEVs triggered cytotoxicity, and mitophagy in the HT-29 carcinoid cells, channelized through the Akt/mTOR pathways connecting the cellular oxidative stress, effectively played its part to cause cells death. These findings substantiated the
BEVs\' potential as a plausible tool for treating, and possibly preventing the colorectal cancer.