%0 Journal Article
%T TCID50 Assay: A Simple Method to Determine Baculovirus Titer.
%A Zhang Z
%A Zhang X
%A Chen H
%J Methods Mol Biol
%V 2829
%N 0
%D 2024
%M 38951342
暂无%R 10.1007/978-1-0716-3961-0_20
%X There are many methods that can be used to determine the infectious titer of your baculovirus stock. The TCID50 method is a simple end-point dilution method that determines the amount of baculovirus virus needed to produce a cytopathic effect or kill 50% of inoculated insect cells. Serial dilutions of baculovirus stock are added to Sf9 cells cultivated in 96-well plates and 3-5 days after infection, cells are monitored for cell death or cytopathic effect. The titer can then be calculated by the Reed-Muench method as described in this method.