艾蒿是一种传统的草药植物,由于艾灸在全球范围内的应用,其艾蒿叶的需求量很大。生长素/吲哚-3-乙酸(Aux/IAA,或IAA)基因家族在主要的生长素反应过程中具有关键作用,广泛参与植物发育和胁迫,控制植物的各种基本性状。然而,阿吉Aux/IAA基因家族的系统研究仍然有限。在这项研究中,总共对61个Aux/IAA基因进行了全面鉴定和表征。基因结构分析表明,46个Aux/IAA蛋白含有4个典型结构域,Aux/IAA蛋白属于非规范IAA蛋白。共线预测和系统发育关系分析表明,Aux/IAA蛋白分为13个不同的类别,大多数Aux/IAA基因在串联复制过程中可能会经历基因丢失。启动子顺式元件研究表明,Aux/IAA启动子含有多种植物激素反应和胁迫反应顺式元件。蛋白质相互作用预测分析表明AaIAA26/29/7/34蛋白质可能是Aux/IAA家族相互作用的核心成员。通过RNA-seq数据在根和叶中的表达分析表明,一些AaIAAs的表达表现出组织特异性表达模式,一些AaIAAs参与了盐和盐碱胁迫的调节。此外,RT-qPCR结果表明,AaIAA基因对生长素的反应具有差异,对其他激素有复杂的反应模式,表明Aux/IAA可能在连接生长素和其他激素信号通路中发挥作用。总的来说,这些发现进一步阐明了AaIAA基因,并为阐明其在植物生长过程中的功能提供了关键的基础知识,应激反应,和Aux/IAA家族基因的激素网络。
Artemisia argyi is a traditional herbal medicine plant, and its folium artemisia argyi is widely in demand due to moxibustion applications globally. The Auxin/indole-3-acetic acid (Aux/IAA, or IAA) gene family has critical roles in the primary auxin-response process, with extensive involvement in plant development and stresses, controlling various essential traits of plants. However, the systematic investigation of the Aux/IAA gene family in A. argyi remains limited. In this study, a total of 61 Aux/IAA genes were comprehensively identified and characterized. Gene structural analysis indicated that 46 Aux/IAA proteins contain the four typical domains, and 15 Aux/IAA proteins belong to non-canonical IAA proteins. Collinear prediction and phylogenetic relationship analyses suggested that Aux/IAA proteins were grouped into 13 distinct categories, and most Aux/IAA genes might experience gene loss during the tandem duplication process. Promoter cis-element investigation indicated that Aux/IAA promoters contain a variety of plant hormone response and stress response cis-elements. Protein interaction prediction analysis demonstrated that AaIAA26/29/7/34 proteins are possibly core members of the Aux/IAA family interaction. Expression analysis in roots and leaves via RNA-seq data indicated that the expression of some AaIAAs exhibited tissue-specific expression patterns, and some AaIAAs were involved in the regulation of salt and saline-alkali stresses. In addition, RT-qPCR results indicated that AaIAA genes have differential responses to auxin, with complex response patterns in response to other hormones, indicating that Aux/IAA may play a role in connecting auxin and other hormone signaling pathways. Overall, these findings shed more light on AaIAA genes and offer critical foundational knowledge toward the elucidation of their function during plant growth, stress response, and hormone networking of Aux/IAA family genes in A. argyi.