PDF(Pubmed)
Abstract：
The auxin/indole-3-acetic acid (Aux/IAA) and auxin response factor (ARF) genes are two crucial gene families in the plant auxin signaling pathway. Nonetheless, there is limited knowledge regarding the Aux/IAA and ARF gene families in Populus simonii. In this study, we first identified 33 putative PsIAAs and 35 PsARFs in the Populus simonii genome. Analysis of chromosomal location showed that the PsIAAs and PsARFs were distributed unevenly across 17 chromosomes, with the greatest abundance observed on chromosomes 2. Furthermore, based on the homology of PsIAAs and PsARFs, two phylogenetic trees were constructed, classifying 33 PsIAAs and 35 PsARFs into three subgroups each. Five pairs of PsIAA genes were identified as the outcome of tandem duplication, but no tandem repeat gene pairs were found in the PsARF family. The expression profiling of PsIAAs and PsARFs revealed that several genes exhibited upregulation in different tissues and under various stress conditions, indicating their potential key roles in plant development and stress responses. The variance in expression patterns of specific PsIAAs and PsARFs was corroborated through RT-qPCR analysis. Most importantly, we instituted that the PsIAA7 gene, functioning as a central hub, exhibits interactions with numerous Aux/IAA and ARF proteins. Furthermore, subcellular localization findings indicate that PsIAA7 functions as a protein localized within the nucleus. To conclude, the in-depth analysis provided in this study will contribute significantly to advancing our knowledge of the roles played by PsIAA and PsARF families in both the development of P. simonii tissue and its responses to stress. The insights gained will serve as a valuable asset for further inquiries into the biological functions of these gene families.
摘要：
生长素/吲哚-3-乙酸(Aux/IAA)和生长素响应因子(ARF)基因是植物生长素信号通路中的两个关键基因家族。尽管如此，关于杨树Aux/IAA和ARF基因家族的知识有限。在这项研究中,我们首先在胡杨基因组中鉴定了33个推定的PsIAAs和35个PsARFs。染色体定位分析表明,PsIAAs和PsARFs在17条染色体上分布不均,在2号染色体上观察到的丰度最大。此外，基于PsIAAs和PsARFs的同源性，构建了两棵系统发育树，将33个PsIAAs和35个PsARFs分为三个亚组。5对PsIAA基因被鉴定为串联复制的结果，但是在PsARF家族中没有发现串联重复基因对。PsIAAs和PsARFs的表达谱显示，几个基因在不同组织和不同胁迫条件下表现出上调。表明它们在植物发育和胁迫反应中的潜在关键作用。通过RT-qPCR分析证实了特异性PsIAA和PsARF的表达模式的差异。最重要的是,我们建立了PsIAA7基因，作为中央枢纽，表现出与许多Aux/IAA和ARF蛋白的相互作用。此外，亚细胞定位结果表明，PsIAA7作为位于细胞核内的蛋白质发挥作用。最后，本研究提供的深入分析将有助于提高我们对PsIAA和PsARF家族在西蒙氏疟原虫组织发育及其对应激反应中所起作用的认识.获得的见解将为进一步调查这些基因家族的生物学功能提供宝贵的资产。
