固体平板已用于微生物单克隆分离,耕种,以及1881年以来的殖民地采摘。然而,该过程是劳动力和资源密集型的高通量要求。目前,已经集成了几种仪器,用于自动化和高通量的采摘,但又复杂又昂贵.为了解决这些问题,我们报告了一个新颖的集成平台,单细胞微升液滴筛选系统(MISS细胞),对于自动化,高通量微生物单克隆菌落培养和采摘。我们验证了MISS细胞中液滴培养物的单克隆性并表征了培养性能。与实心板相比,MISS细胞使用更少的资源产生了更多数量的单克隆集落,初始生长速率更高。最后,我们建立了使用MISS细胞自动化高通量筛选谷氨酸棒杆菌的工作流程,并鉴定了高谷氨酸生产菌株.MISS细胞可以作为一个通用平台,在高通量应用中高效生产单克隆菌落,克服固体板的局限性,促进生物技术的快速发展。本文受版权保护。保留所有权利。
Solid plates have been used for microbial monoclonal isolation, cultivation, and colony picking since 1881. However, the process is labor- and resource-intensive for high-throughput requirements. Currently, several instruments have been integrated for automated and high-throughput picking, but complicated and expensive. To address these issues, we report a novel integrated platform, the single-cell microliter-droplet screening system (MISS Cell), for automated, high-throughput microbial monoclonal colony cultivation and picking. We verified the monoclonality of droplet cultures in the MISS Cell and characterized culture performance. Compared with solid plates, the MISS Cell generated a larger number of monoclonal colonies with higher initial growth rates using fewer resources. Finally, we established a workflow for automated high-throughput screening of Corynebacterium glutamicum using the MISS Cell and identified high glutamate-producing strains. The MISS Cell can serve as a universal platform to efficiently produce monoclonal colonies in high-throughput applications, overcoming the limitations of solid plates to promote rapid development in biotechnology.