亚洲梨(Pyrusbretschneideri)是世界上最重要的水果作物之一,其生长和生产力经常受到非生物胁迫的影响。钙调神经磷酸酶B样相互作用蛋白激酶(CIPK)作为钙传感器蛋白激酶与结合Ca(2)的CBL相互作用,以广泛介导植物的非生物胁迫反应。虽然梨的基因组序列已经发布,关于梨的CIPK基因的信息很少,尤其是对盐和渗透压力的反应。在这项研究中,我们从测序的梨基因组中系统地鉴定了28CIPK家族成员,并分析了它们的组织,系统发育,基因结构,蛋白质基序,和同义重复差异。大多数重复的PbCIPK都经过了纯化选择,它们的进化差异伴随着梨的全基因组复制。我们还研究了盐和渗透胁迫下CIPK家族的应激反应表达模式和共表达网络,以及与应激相关的顺式调控元件在启动子区的分布。我们的结果表明,大多数PbCIPKs可能在非生物胁迫反应中起重要作用。一些PbCIPKs,例如PbCIPK22、-19、-18、-15、-8和-6可以基于PbCIPKs的共表达网络作为响应盐和渗透胁迫的核心调节剂。一些参与盐反应的基因与那些参与渗透反应的基因没有重叠,表明CIPK基因在应激反应中的亚功能化。这项研究揭示了一些候选基因在对盐和渗透胁迫的早期反应中起作用,以进一步表征梨中CIPKs的非生物胁迫反应。
Asian pear (Pyrus bretschneideri) is one of the most important fruit crops in the world, and its growth and productivity are frequently affected by abiotic stresses. Calcineurin B-like interacting protein kinases (CIPKs) as caladium-sensor protein kinases interact with Ca(2+)-binding CBLs to extensively mediate abiotic stress responses in plants. Although the pear genome sequence has been released, little information is available about the CIPK genes in pear, especially in response to salt and osmotic stresses. In this study, we systematically identified 28 CIPK family members from the sequenced pear genome and analyzed their organization, phylogeny, gene structure, protein motif, and synteny duplication divergences. Most duplicated PbCIPKs underwent purifying selection, and their evolutionary divergences accompanied with the pear whole genome duplication. We also investigated stress -responsive expression patterns and co-expression networks of CIPK family under salt and osmotic stresses, and the distribution of stress-related cis-regulatory elements in promoter regions. Our results suggest that most PbCIPKs could play important roles in the abiotic stress responses. Some PbCIPKs, such as PbCIPK22, -19, -18, -15, -8, and -6 can serve as core regulators in response to salt and osmotic stresses based on co-expression networks of PbCIPKs. Some sets of genes that were involved in response to salt did not overlap with those in response to osmotic responses, suggesting the sub-functionalization of CIPK genes in stress responses. This study revealed some candidate genes that play roles in early responses to salt and osmotic stress for further characterization of abiotic stress responses medicated by CIPKs in pear.