背景:尤卡坦半岛拥有丰富的维管植物,已开发出各种玛雅草药配方。然而,关于它们的抗致病性和抗毒力特性的研究很少。
目的:确定玛雅草药的抗毒力特性,并确定其在感染铜绿假单胞菌的烧伤创面中的抗病原能力。
方法:在金塔纳罗奥州中部和南部的玛雅社区进行了民族植物学研究,墨西哥。此外,使用热损伤和铜绿假单胞菌感染的动物模型分析了三种玛雅草药的抗致病性。通过抑制群体感应调节的表型来确定抗毒力特性(绿脓苷,生物膜,和蜂拥而至),并通过ExoU毒素的分泌。使用分子网络分析分析了最活跃的草药的化学成分。
结果:发现局部施用称为“草药皂”(HS)的药物11天可以维持动物100%的存活率,减少烧伤中细菌的建立并防止其全身扩散。虽然对HS治疗损伤的组织没有疗效记录,其草药成分强烈减少蜂群和ExoU分泌。通过对分子网络的分析,有可能对其化学成分进行全球研究,并鉴定了羟吲哚单萜生物碱和咔啉和四氢嘧啶生物碱的家族。此外,黄酮醇,黄烷-3-醇,并检测到奎尼酸衍生物。
结论:古代玛雅疗法的抗致病性和抗毒性能力使其成为开发新的抗菌疗法以治疗铜绿假单胞菌感染的烧伤的潜在资源。
BACKGROUND: The Yucatan Peninsula has a privileged wealth of vascular plants with which various Mayan herbal formulations have been developed. However, studies on their antipathogenic and
antivirulence properties are scarce.
OBJECTIVE: Identify
antivirulence properties in Mayan herbal remedies and determine their antipathogenic capacity in burn wounds infected with Pseudomonas aeruginosa.
METHODS: An ethnobotanical study was conducted in Mayan communities in central and southern Quintana Roo, Mexico. Furthermore, the antipathogenic capacity of three Mayan herbal remedies was analyzed using an animal model of thermal damage and P. aeruginosa infection.
Antivirulence properties were determined by inhibiting phenotypes regulated by quorum sensing (pyocyanin, biofilm, and swarming) and by the secretion of the ExoU toxin. The chemical composition of the most active herbal remedy was analyzed using molecular network analysis.
RESULTS: It was found that topical administration of the remedy called \"herbal soap\" (HS) for eleven days maintained 100% survival of the animals, reduced establishment of the bacteria in the burn and prevented its systemic dispersion. Although no curative effect was recorded on tissue damaged by HS treatment, its herbal composition strongly reduced swarming and ExoU secretion. Through analysis of Molecular Networks, it was possible to carry out a global study of its chemical components, and identify the family of oxindole monoterpenoid alkaloids and carboline and tetrahydropyrididole alkaloids. In addition, flavonols, flavan-3-ols, and quinic acid derivatives were detected.
CONCLUSIONS: The antipathogenic and
antivirulence capacity of ancient Mayan remedies makes them a potential resource for developing new antibacterial therapies to treat burns infected by P. aeruginosa.